...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Agricultural and Food Chemistry >Extraction and Analysis of Auxins in Plants Using Dispersive Liquid-Liquid Microextraction Followed by High-Performance Liquid Chromatography with Fluorescence Detection
【24h】

Extraction and Analysis of Auxins in Plants Using Dispersive Liquid-Liquid Microextraction Followed by High-Performance Liquid Chromatography with Fluorescence Detection

机译：分散液-液微萃取-高效液相色谱-荧光检测法提取植物中的生长素

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Auxin plays an important role in cell differentiation, apical dominance, and tropism in plants. A new method based on dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography-fluorescence detection (HPLC-FLD) has been established to detect auxin. A mixture of CHCl3 (extraction solvent) and acetone (disperser solvent) was injected quickly into a sample solution with desired salt concentration and pH value, and then a cloudy solution consisting of many dispersed fine droplets of CHCl3 was formed. After centrifugation, the sedimented phase was withdrawn and directly analyzed by HPLC-FLD. Under optimal conditions, four auxins were baseline separated within 3.5 min, with the minimal limit of detection of 0.02 ng mL~(-1) and coefficient correlations in the range of 0.9980-0.9995. This simple method was successfully applied to real sample analysis. Experimental results showed that DLLME was a high-performance and powerful preconcentration method to extract and enrich related plant auxin.
机译：生长素在植物的细胞分化，顶端优势和向性中起重要作用。建立了基于分散液-液微萃取（DLLME）结合高效液相色谱-荧光检测（HPLC-FLD）检测植物生长素的新方法。将CHCl3（萃取溶剂）和丙酮（分散剂溶剂）的混合物快速注入具有所需盐浓度和pH值的样品溶液中，然后形成由许多分散的CHCl3细滴组成的混浊溶液。离心后，取出沉淀相并通过HPLC-FLD直接分析。在最佳条件下，在3.5分钟内将四个生长素基线分离，最小检出限为0.02 ng mL〜（-1），系数相关性在0.9980-0.9995范围内。此简单方法已成功应用于实际样品分析。实验结果表明，DLLME是提取和富集相关植物生长素的一种高性能，强大的预浓缩方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号