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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Optimization of Adsorption of Tea Polyphenols into Oat β-GIucan Using Response Surface Methodology
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Optimization of Adsorption of Tea Polyphenols into Oat β-GIucan Using Response Surface Methodology

机译：响应面法优化燕麦多酚对茶多酚的吸附

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摘要

Information on interactions between oat β-glucan and tea polyphenols (TP) is not available in the published literatures. Equilibrium dialysis was applied to determine the adsorption of TP into ,8-glucan, and response surface methodology (RSM) was employed to optimize the absorbing variables (pH, temperature, and phosphate buffered saline (PBS) buffer concentration). The equilibrium data at constant temperature were fitted with the Langmuir, Freundlich, and Redlich-Peterson models. The results showed that the Freundlich model was the best method to describe the experimental data. Parabolic curves were obtained for pH and temperature. In terms of adsorption capacity, factors including temperature, pH/temperature, and buffer concentration/temperature had the greatest influence on the response. The highest adsorption capacity of TP into β-glucan was 134.55 μg mg~(-1) at the following optimized conditions: pH 5.56, PBS buffer concentration 0.13 M, and temperature 40 °C. No significant differences (p > 0.05) between the experimental and predicted values confirmed the adequacy of the response surface equations.
机译：燕麦β-葡聚糖与茶多酚（TP）之间相互作用的信息在已发表的文献中不可用。应用平衡渗析法确定TP在1,8-葡聚糖中的吸附，并采用响应面法（RSM）优化吸收变量（pH，温度和磷酸盐缓冲盐水（PBS）缓冲液浓度）。用Langmuir，Freundlich和Redlich-Peterson模型拟合恒温条件下的平衡数据。结果表明，Freundlich模型是描述实验数据的最佳方法。获得了pH和温度的抛物线曲线。就吸附能力而言，温度，pH /温度和缓冲液浓度/温度等因素对响应的影响最大。在以下优化条件下：pH 5.56，PBS缓冲液浓度0.13 M，温度40°C，TP对β-葡聚糖的最大吸附量为134.55μgmg〜（-1）。实验值和预测值之间没有显着差异（p> 0.05），确认了响应面方程的适当性。

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