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首页> 外文期刊>Journal of Agricultural and Food Chemistry >High Molecular Weight Persimmon (Diospyros kaki L.) Proanthocyanidin: A Highly Galloylated, A-Linked Tannin with an Unusual Flavonol Terminal Unit, Myricetin
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High Molecular Weight Persimmon (Diospyros kaki L.) Proanthocyanidin: A Highly Galloylated, A-Linked Tannin with an Unusual Flavonol Terminal Unit, Myricetin

机译：高分子量柿子（Diospyros kaki L.）原花青素：高度镀铝的，A链单宁，具有不常见的黄酮醇末端单元，杨梅素

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摘要

MALDI-TOF MS suggested that the high molecular weight proanthocyanidin (condensed tannin) from persimmon (Diospyros kaki L.) pulp comprised a heteropolyflavanol series with flavan-3-O-galloylated extenders, flavan-3-ol and flavonol terminal units, and A-type interflavan linkages. Thiolysis-HPLC-ESI-MS with DAD, electrochemical, and ESI-MS detection confirmed a previously unreported terminal unit, the flavonol myricetin, in addition to the typical flavan-3-ols catechin and epigallocatechin gallate. The extender units were epicatechin, epigallocatechin, (epi)gallocatechin-3-O-gallate, and (epi)catechin-3-O-gallate. The crude tannin had a high prodelphihidin content (65%) and a high degree of 3-Ogalloylation (72%). The material was fractionated on Toyopearl TSK-HW-50-F to yield fractions distinguished by degree of polymerization (DP). Thiolysis suggested'that the persimmon tannin was composed of polymers ranging from 7 to 20 kDa (DP 19-47), but sizes estimated by GPC were 50-70% smaller. The crude material was chemically degraded with acid to yield products that were amenable to NMR and ESI-MS analysis, which were used to establish for the first time that persimmon tannin has a mixture of B-type and A-type linkages.
机译：MALDI-TOF MS表明，柿子（Diospyros kaki L.）果肉中的高分子量原花青素（缩合单宁）包含杂多黄烷醇系列，其中含有flavan-3-O-半烷基化增量剂，flavan-3-ol和flavonol末端单元，以及A型黄酮键。具有DAD的硫解HPLC-ESI-MS，电化学检测和ESI-MS检测证实，除了典型的flavan-3-ols儿茶素和表没食子儿茶素没食子酸酯外，黄酮杨梅素还没有报道过。增量剂单元是表儿茶素，表没食子儿茶素，（表）gallocatechin-3-O-没食子酸酯和（表儿茶素-3-O-没食子酸酯）。粗制丹宁具有较高的原翠鸟苷含量（65％）和较高的3-Oloyloyation度（72％）。将该物质在Toyopearl TSK-HW-50-F上分馏，得到以聚合度（DP）区分的馏分。硫解表明'柿单宁由7至20 kDa（DP 19-47）的聚合物组成，但GPC估计的尺寸要小50-70％。粗物质用酸化学降解，得到的产物适用于NMR和ESI-MS分析，该产物首次用于确定柿子单宁具有B型和A型键的混合物。

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