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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Isolation and Identification of Sea Buckthorn (Hippophae rhamnoides) Phenolics with Antioxidant Activity and α-Glucosidase Inhibitory Effect
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Isolation and Identification of Sea Buckthorn (Hippophae rhamnoides) Phenolics with Antioxidant Activity and α-Glucosidase Inhibitory Effect

机译：具有抗氧化活性和α-葡萄糖苷酶抑制作用的沙棘酚类化合物的分离与鉴定

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摘要

This study was performed to evaluate the antioxidant and α-glucosidase inhibitory effects from the extract, fractions, and isolated compounds of sea buckthorn leaves. Six compounds, kaempferol-3-O-β-D-(6"-O-coumaryl) glycoside, 1-feruloyl-β-D-glucopyranoside, isorhamnetin-3-Oglucoside, quercetin 3-O-β-D-glucopyranoside, quercetin 3-O-β-D-gluropyranosyl-7-O-α-L-rhamnopyranoside, and isorhamnetin-3-O-rutinoside, were isolated from sea buckthorn leaf extracts. The butanol fraction (EC_(50) = 1.81 μg/ mL) along with quercetin 3-O-β-D-glucopyranoside (EC_(50) = 1.86 μg/mL) had a higher DPPH radical-scavenging activity and showed stronger reducing power (OD_(700) = 1.83 and 1.78, respectively). The butanol fraction (477 mg GAE/g) contained the highest amount of phenolic compounds and also the most powerful a-glucosidase inhibitory effect (86%) at 5 μg/mL The results indicate that sea buckthorn leaf extracts could potentially be used for food additives and the development of useful natural compounds.
机译：进行这项研究以评估沙棘叶提取物，馏分和分离出的化合物的抗氧化剂和α-葡萄糖苷酶抑制作用。六种化合物，kaempferol-3-O-β-D-（6“ -O-香豆基）糖苷，1-阿魏酰-β-D-吡喃葡萄糖苷，异鼠李素-3-O葡萄糖苷，槲皮素3-O-β-D-吡喃葡萄糖苷，从沙棘叶提取物中分离出槲皮素3-O-β-D-葡萄糖吡喃糖基-7-O-α-L-鼠李吡喃糖苷和异鼠李素-3-O-芸香糖苷，丁醇含量（EC_（50）= 1.81μg/ mL）和槲皮素3-O-β-D-吡喃葡萄糖苷（EC_（50）= 1.86μg/ mL）具有更高的DPPH自由基清除活性并显示出更强的还原力（OD_（700）= 1.83和1.78） 。当浓度为5μg/ mL时，丁醇级分（477 mg GAE / g）含有最高量的酚类化合物和最强大的α-葡萄糖苷酶抑制作用（86％）。结果表明，沙棘叶提取物可用于食品添加剂和有用的天然化合物的开发。

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