...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Agricultural and Food Chemistry >Simultaneous Production of Trehalose, Bioethanol, and High-Protein Product from Rice by an Enzymatic Process
【24h】

Simultaneous Production of Trehalose, Bioethanol, and High-Protein Product from Rice by an Enzymatic Process

机译：通过酶法同时从水稻生产海藻糖，生物乙醇和高蛋白产品

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rice is a starch-rich raw material that can be used for trehalose production. It can be hydrolyzed with α-amylase, β-amylase, and pullulanase to produce high-maltose content of rice saccharified solution for bioconversion of maltose into trehalose by trehalose synthase (TSase). For this purpose, an efficient enzymatic procedure has been successfully developed to simultaneously produce value-added trehalose, bioethanol, and high-protein product from rice as substrate. The highest maltose yield produced from the liquefied rice starch hydrolysate was 82.4 ± 2.8% at 50 °C and pH 5.0 for 21 -22 h. The trehalose conversion rate can reach at least 50% at 50 °C and pH 5.0 for 20-24 h by a novel thermostable recombinant Picrophilus torridus trehalose synthase (PTTS). All residual sugar, except trehalose, can be fully hydrolyzed by glucoamylase into glucose for further bioethanol production. The insoluble byproduct containing high yields of protein (75.99%) and dietary fiber (14.01%) can be processed as breakfast cereal product, health food, animal forage, etc. The conversion yield of bioethanol was about 98% after 64 h of fermentation time by Sacchar-omyces cerevisiae without any artificial culture solution addition. Ethanol can easily be separated from trehalose by distillation with a high recovery yield and purity of crystalline trehalose of 92.5 ± 8.7% and 92.3%, respectively.
机译：大米是富含淀粉的原料，可用于生产海藻糖。它可以用α-淀粉酶，β-淀粉酶和支链淀粉酶水解，以产生高麦芽糖含量的大米糖化溶液，用于通过海藻糖合酶（TSase）将麦芽糖生物转化为海藻糖。为此目的，已经成功开发了一种有效的酶促程序，可同时从大米作为底物生产增值的海藻糖，生物乙醇和高蛋白产品。在50°C和pH 5.0的条件下，液化米淀粉水解物产生的最高麦芽糖产量为82.4±2.8％，持续21 -22 h。通过一种新型的热稳定的重组拟南芥（Pitrophilus torridus）海藻糖合酶（PTTS），在50°C和pH 5.0下海藻糖转化率可以达到至少50％，持续20-24小时。除海藻糖外，所有残留的糖都可以被葡糖淀粉酶完全水解为葡萄糖，以进一步生产生物乙醇。可以将高蛋白（75.99％）和膳食纤维（14.01％）的不溶性副产物加工成早餐谷物产品，保健食品，动物饲料等。发酵64小时后，生物乙醇的转化率约为98％由酿酒酵母（Sacchar-omyces cerevisiae）制备，无需添加任何人工培养液。乙醇可以很容易地通过蒸馏从海藻糖中分离出来，结晶海藻糖的回收率和纯度分别高达92.5±8.7％和92.3％。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号