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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Evaluation of Two Derivatization Reagents for the Determination by LC-MS/MS of Ammonia in Cigarette Mainstream Smoke
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Evaluation of Two Derivatization Reagents for the Determination by LC-MS/MS of Ammonia in Cigarette Mainstream Smoke

机译：LC-MS / MS法测定卷烟主流烟气中氨的两种衍生试剂的评价

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摘要

Ammonia in cigarette mainstream smoke was quantified by LC-MS/MS after derivatization. Two different reagents, fluorescamine and dansyl chloride, were investigated, but only the latter gave stable derivatives; therefore, it was considered the most appropriate choice. Smoke samples were collected on a Cambridge filter pad followed by an impinger containing a solution of hydrochloric acid. Ammonia was then derivatized with a 18.5 mM solution of dansyl chloride in acetonitrile at 70 °C for 30 min in a vial with the internal standard, ~(15)ND4Cl. The resulting derivative was analyzed by LC-MS/MS detection with an atmospheric pressure chemical ionization (APCl) interface in the positive ionization mode using multiple-reaction monitoring (MRM). Good linearity was obtained in the concentration range of 0.02-1,65 ,μg/mL (r~2≥ 0.999), and the limit of detection (LOD) was established at 0.006 μg/mL. This method has the advantage of being sensitive, efficient, and reliable and is not hindered by interferences from the sample matrix. It should thus be considered a reference method of choice for the determination of ammonia in smoke.
机译：衍生后，通过LC-MS / MS对卷烟主流烟气中的氨进行定量。研究了两种不同的试剂，氟胺和丹磺酰氯，但只有后者能提供稳定的衍生物。因此，它被认为是最合适的选择。在剑桥滤垫上收集烟雾样品，然后在装有盐酸溶液的冲击器上收集。然后在内标〜（15）ND4Cl的小瓶中，在70°C下用18.5 mM的丹磺酰氯在乙腈中的溶液将氨衍生30分钟。通过多反应监测（MRM）在正电离模式下使用大气压化学电离（APCl）接口通过LC-MS / MS检测对所得衍生物进行分析。浓度范围为0.02-1,65μg/ mL（r〜2≥0.999）时，线性良好，检出限（LOD）为0.006μg/ mL。该方法具有灵敏，有效和可靠的优点，并且不受样品基质干扰的阻碍。因此，应将其视为测定烟雾中氨气的参考选择方法。

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