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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Loop-Mediated Isothermal Amplification (LAMP): Methods for Plant Species Identification in Food
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Loop-Mediated Isothermal Amplification (LAMP): Methods for Plant Species Identification in Food

机译：回路介导的等温扩增（LAMP）：食品中植物物种鉴定的方法

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Loop-mediated isothermal amplification (LAMP) is a DNA-based analytical method that can be used as an isothermal alternative to polymerase chain reaction (PCR). In comparison to PCR, the advantage of LAMP is the possibility to perform the isothermal reaction without any sophisticated technical equipment; only a water bath is needed, and naked eye detection is sufficient. Up to now, an application of LAMP methods for the detection of even closely related plant species in food or feed matrices has not been described, whereas a large number of PCR methods for that topic are cited in the literature. The aim of the study was the evaluation of LAMP-based methods for plant species identification with respect to method parameters such as R~2, LOD, and LOQ. An existing (real-time) PCR method (for the detection of spices) was used for comparison. It could be shown that the developed LAMP methods have potential as alternative strategies to PCR in DNA-based analysis.
机译：环介导的等温扩增（LAMP）是一种基于DNA的分析方法，可以用作聚合酶链反应（PCR）的等温替代方法。与PCR相比，LAMP的优势在于无需任何复杂的技术设备即可进行等温反应。只需要一个水浴，肉眼检测就足够了。迄今为止，尚未描述将LAMP方法用于检测食品或饲料基质中甚至密切相关的植物物种的方法，而文献中引用了大量针对该主题的PCR方法。这项研究的目的是评估基于LAMP的植物物种识别方法，如方法参数R〜2，LOD和LOQ。使用现有的（实时）PCR方法（用于检测香料）进行比较。可以证明，已开发的LAMP方法有潜力作为基于DNA的分析中PCR的替代策略。

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