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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Metabolic Profiling of Transgenic Potato Tubers Expressing Arabidopsis Dehydration Response Element-Binding Protein 1A (DREB1A)
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Metabolic Profiling of Transgenic Potato Tubers Expressing Arabidopsis Dehydration Response Element-Binding Protein 1A (DREB1A)

机译:表达拟南芥脱水反应元件结合蛋白1A(DREB1A)的转基因马铃薯块茎的代谢谱分析。

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摘要

Untargeted metabolome analyses play a critical role in understanding possible metabolic fluctuations of crops under varying environmental conditions. This study reports metabolic profiles of transgenic potato tubers expressing the Arabidopsis DREB1A transcription factor gene, which induces expression of genes involved in environmental stress tolerance. A combination of targeted and untargeted metabolomics demonstrated considerable metabolome differences between the transgenic lines and nontransgenic parent cultivars. In the transgenic lines, stimulation of stress responses was suggested by elevated levels of the glutathione metabolite, γ-aminobutyric acid (GABA), and by the accumulation of β-cyanoalanine, a byproduct of ethylene biosynthesis. These results suggest that the Arabidopsis DREB1A expression might directly or indirectly enhance endogenous potato stress tolerance systems. The results indicate that transgenesis events could alter the metabolic compositions in food crops, and therefore metabolomics analysis could be a most valuable tool to monitor such changes.
机译:非目标代谢组分析在理解环境条件不同时农作物可能的代谢波动中起着至关重要的作用。这项研究报告了表达拟南芥DREB1A转录因子基因的转基因马铃薯块茎的代谢概况,该基因诱导了环境胁迫耐受性相关基因的表达。靶向和非靶向代谢组学的组合表明,转基因品系和非转基因亲本品种之间存在相当大的代谢组差异。在转基因品系中,谷胱甘肽代谢产物γ-氨基丁酸(GABA)的水平升高以及乙烯生物合成的副产物β-氰基丙氨酸的积累表明刺激应激反应。这些结果表明拟南芥DREB1A表达可能直接或间接增强内源马铃薯胁迫耐受性系统。结果表明转基因事件可能会改变粮食作物的代谢组成,因此代谢组学分析可能是监测此类变化的最有价值的工具。

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