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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Structural identification of Theaflavin Trigallate and Tetragallate from Black Tea Using Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry
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Structural identification of Theaflavin Trigallate and Tetragallate from Black Tea Using Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry

机译：液相色谱/电喷雾串联质谱法从红茶中茶黄素三没食子酸酯和四没食子酸酯的结构鉴定

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摘要

Black tea contains two major pigments, theaflavins and thearubigins. These polyphenols have been associated with certain health benefits including prevention of heart disease and cancer. Elucidating and characterizing the structural aspects of thearubigins, the most abundant pigmentin black tea, has been a challenge for many years. Therefore farther studies of black tea polyphenols must be conducted in effort to solve this thearubigin dispute. In the present study, black tea extract was found to possess theaflavin trigallate and tetragallate by means of liquid chromatography/electrospray ionization mass spectrometry. These structures were confirmed by analysis of the MS~n (n = 1-4) spectra and comparison of the MS/MS spectra of the product ions to the MS/MS spectra of authentic (-)-epigallocatechin-3-gallate, (-)-epicatechin-3-gallate and theaflavin-3,3'-digallate. To our knowledge, this is the first report to confirm the presence of theaflavin trigallate and tetragallate in black tea.
机译：红茶包含两种主要色素，茶黄素和茶红素。这些多酚与某些健康益处有关，包括预防心脏病和癌症。阐明和表征茶红素（红茶中最丰富的色素）的结构方面一直是一项挑战。因此，必须进一步研究红茶中的多酚以解决这种茶红素的争议。在本研究中，通过液相色谱/电喷雾电离质谱法发现红茶提取物具有茶黄素三没食子酸酯和四没食子酸酯。这些结构通过分析MS〜n（n = 1-4）光谱以及将产物离子的MS / MS光谱与真实的（-）-epigallocatechin-3-gallate的MS / MS光谱进行比较来确认，（ -）-表儿茶素-3-没食子酸酯和茶黄素-3,3'-戊二酸酯。据我们所知，这是第一个证实茶黄素三没食子酸酯和四没食子酸酯存在的报告。

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