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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Trichoderma harzianum ETS 323-Mediated Resistance in Brasska oleracea var. capitata to Rhizoctonia solani Involves the Novel Expression of a Glutathione S-Transferase and a Deoxycytidine Deaminase
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Trichoderma harzianum ETS 323-Mediated Resistance in Brasska oleracea var. capitata to Rhizoctonia solani Involves the Novel Expression of a Glutathione S-Transferase and a Deoxycytidine Deaminase

机译：哈氏木霉ETS 323介导的甘蓝型油菜抗性。到茄枯萎病菌涉及谷胱甘肽S-转移酶和脱氧胞苷脱氨酶的新型表达

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摘要

Plant interactions with microbial biocontrol agents are used as experimental models to understand resistance-related molecular adaptations of plants. In a hydroponic three-way interaction study, a novel Trichoderma harzianum ETS 323 mediated mechanism was found to induce resistance to Rhizoctonia solani infection in Brasska oleracea var. capitata plantlets. The R. solani challenge on leaves initiate an increase in lipoxygenase activity and associated hypersensitive tissue damage with characteristic "programmed cell death" that facilitate the infection. However, B, oleracea plantlets whose roots were briefly (6 h) coloni2ed by T. harzianum ETS 323 developed resistance to R. solani infection through a significant reduction of the host hypersensitive tissue damage. The resistance developed in the distal leaf tissue was associated with the expression of a H2O2-inducible glutathione S-transferase (BoGST), which scavenges cytotoxic reactive electrophiles, and of a deoxycytidine deaminase (BoDCD), which modulates the host molecular expression and potentially neutralizes the DNA adducts and maintains DNA integrity. The cDNAs of BoGST and BoDCD were cloned and sequenced; their expressions were verified by reverse-transcription polymerase chain reaction analysis and were found to be transcriptionally activated during the three-way interaction.
机译：植物与微生物生物防治剂的相互作用被用作实验模型，以了解与植物抗性相关的分子适应性。在一项水耕三效相互作用研究中，发现了一种新的哈茨木霉ETS 323介导的机制，可诱导油菜变种对茄红枯菌的抗药性。人头苗。叶片上的茄枯萎病菌挑战引发脂氧合酶活性的增加和相关的过敏性组织损伤，并伴有有助于感染的特征性“程序性细胞死亡”。然而，其根被哈茨木霉ETS 323短暂地（6小时）定植的油菜B小植株通过显着减少宿主超敏组织损伤而对茄茄R. solani感染产生了抗性。在远端叶片组织中产生的抗性与清除细胞毒性反应性亲电试剂的H2O2诱导型谷胱甘肽S-转移酶（BoGST）和脱氧胞苷脱氨酶（BoDCD）的表达有关，后者调节宿主分子的表达并可能中和。 DNA加合物并保持DNA完整性。 BoGST和BoDCD的cDNA被克隆和测序。通过逆转录聚合酶链反应分析验证了它们的表达，发现它们在三向相互作用中被转录激活。

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