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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Immunochemical Authentication of Manuka Honey Using a Monoclonal Antibody Specific to a Glycoside of Methyl Syringate
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Immunochemical Authentication of Manuka Honey Using a Monoclonal Antibody Specific to a Glycoside of Methyl Syringate

机译：麦卢卡蜂蜜的免疫化学鉴定，其使用的是丁二酸甲酯糖苷特异的单克隆抗体

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摘要

Leptosperin, a novel glycoside of methyl syringate, is exclusively present in manuka honey derived from the Leptospermum species Leptospermum scoparium. Quantification of leptosperin might thus be applicable for authentication of honey. The concentration of leptosperin has high linearity with antibacterial activity. We established a monoclonal antibody to leptosperin and characterized the antibody in detail by a competitive enzyme-linked immunosorbent assay (ELISA), comparing the results with those of the high-performance liquid chromatography (HPLC) method for validation. The antigen in manuka honey was confirmed as leptosperin by HPLC fractionation with quantitation by an ELISA. Leptosperin contents of 50 honey samples were analyzed by an established ELISA, which can handle 20 samples (duplicate) on one 96-well plate. Significant coincidence with the chemical quantitation was observed. Immunochemical quantitation of leptosperin would be an economical and facile method for the possible authentication of manuka honey, allowing many honey samples to be processed and analyzed by an ELISA simultaneously.
机译：Leptosperin是一种新的丁香酸甲酯糖苷，仅存在于来源于Leptospermum物种Leptospermum scoparium的麦卢卡蜂蜜中。因此，瘦素的定量可能适用于蜂蜜的鉴定。瘦素的浓度具有很高的线性和抗菌活性。我们建立了一种针对瘦素的单克隆抗体，并通过竞争性酶联免疫吸附测定（ELISA）对该抗体进行了详细表征，将结果与高效液相色谱（HPLC）方法进行了比较以进行验证。麦卢卡蜂蜜中的抗原通过HPLC分级分离并通过ELISA定量确认为瘦素。通过建立的ELISA分析50个蜂蜜样品中的瘦素含量，该酶标可在一个96孔板上处理20个样品（重复）。观察到与化学定量的显着一致性。瘦肉精的免疫化学定量分析是一种可进行麦卢卡蜂蜜鉴定的经济且简便的方法，可同时处理许多蜂蜜样品并通过ELISA分析。

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