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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Transport of Antihypertensive Peptide RVPSL, Ovotransferrin 328-332, in Human Intestinal Caco-2 Cell Monolayers
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Transport of Antihypertensive Peptide RVPSL, Ovotransferrin 328-332, in Human Intestinal Caco-2 Cell Monolayers

机译：降压肽RVPSL，Ovotransferrin 328-332在人肠Caco-2细胞单层中的转运

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摘要

The objective of this study was to investigate the transepithelial transport of RVPSL (Arg-Val-Pro-Ser-Leu), an egg-white-derived peptide with angiotensin I-converting enzyme (ACE) inhibitory and antihypertensive activity, in human intestinal Caco-2 cell monolayers. Results revealed that RVPSL could be passively transported across Caco-2 cell monolayers. However, during the process of transport, 36.31% +/- 1.22% of the initial RVPSL added to the apical side was degraded, but this degradation decreased to 23.49% +/- 0.68% when the Caco-2 cell monolayers were preincubated with diprotin A (P < 0.001), suggesting that RVPSL had a low resistance to various brush border membrane peptidases. When transport from the apical side to the basolateral side was investigated, the apparent permeability coefficient (P-app) was (6.97 +/- 1.11) x 10(-6) cm/s. The transport route of RVPSL appears to be the paracellular pathway via tight junctions, as only cytochalasin D, a disruptor of tight junctions (TJs), significantly increased the transport rate (P < 0.001). In addition, the relationship between the structure of RVPSL and transport across Caco-2 cell monolayers was studied by mutation of RVPSL. It was found that N-terminal Pro residues were more beneficial for transport of pentapeptides across Caco-2 cell monolayers than Arg and Val. Furthermore, RVPSL could be more easily transported as smaller peptides, especially in the form of dipeptides and tripeptides.
机译：这项研究的目的是调查RVPSL（Arg-Val-Pro-Ser-Leu）的经上皮运输，这是一种蛋白蛋白，具有血管紧张素I转换酶（ACE）抑制和降压活性，在人小肠Caco中的运输-2细胞单层。结果表明，RVPSL可以在Caco-2细胞单层中被动转运。然而，在运输过程中，添加到顶侧的初始RVPSL的36.31％+/- 1.22％被降解，但是当Caco-2细胞单层与双protin一起预孵育时，这种降解降低到23.49％+/- 0.68％。 A（P <0.001），表明RVPSL对各种刷状缘膜肽酶的抵抗力很低。当研究从顶侧到基底外侧的传输时，表观渗透系数（P-app）为（6.97 +/- 1.11）x 10（-6）cm / s。 RVPSL的运输途径似乎是通过紧密连接的细胞旁途径，因为只有细胞松弛素D（紧密连接（TJ）的破坏者）才能显着提高运输速率（P <0.001）。另外，通过RVPSL的突变研究了RVPSL的结构与跨Caco-2细胞单层运输之间的关系。发现N末端Pro残基比Arg和Val更有利于五肽跨Caco-2细胞单层的转运。此外，RVPSL可以更容易地以较小的肽形式运输，尤其是以二肽和三肽形式运输。

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