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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Characterization and Functional Identification of a Gene Encoding Geranylgeranyl Diphosphate Synthase from Dunaliella bardawil
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Characterization and Functional Identification of a Gene Encoding Geranylgeranyl Diphosphate Synthase from Dunaliella bardawil

机译：编码杜氏杜氏藻中香叶基香叶基二磷酸合酶的基因的表征和功能鉴定

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摘要

Geranylgeranyl diphosphate synthase (GGPS) catalyzes the biosynthesis of geranylgeranyl diphosphate, a key precursor for carotenoid biosynthesis. In this study, a full-length cDNA encoding GGPS from Dunaliella bardawil (DbGGPS) was isolated by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of DbGGPS was 1814 bp, containing a 1074 bp ORF encoding 357 amino acids with a calculated mass of 38.88 kDa. Analysis of DbGGPS genomic DNA revealed that it contained 10 exons and 9 introns. It was predicted that DbGGPS possessed a 48 amino acid transit peptide at its N terminus. Bioinformatic analysis revealed that DbGGPS was a member of a group of polyprenyltransferases with five conserved domains and two highly conserved aspartate-rich motifs. Using heterologous expression, carotenoid complementation assay, and gene deletion analysis, it was shown that the coding region of DbGGPS encodes a functional GGPS. This provides new gene sources for carotenoid genetic engineering.
机译：Geranylgeranyl diphosphate合酶（GGPS）催化Geranylgeranyl diphosphate的生物合成，这是类胡萝卜素生物合成的关键前体。在这项研究中，首次通过快速扩增cDNA末端（RACE）分离了来自杜氏杜氏藻（Dunaliella bardawil）的GGPS的全长cDNA（DbGGPS）。 DbGGPS的全长cDNA为1814 bp，包含1074 bp的ORF，编码357个氨基酸，计算质量为38.88 kDa。对DbGGPS基因组DNA的分析显示，它包含10个外显子和9个内含子。据预测，DbGGPS在其N端具有48个氨基酸转运肽。生物信息学分析表明，DbGGPS是具有五个保守结构域和两个高度保守的富含天冬氨酸的基序的聚异戊二烯基转移酶的成员。使用异源表达，类胡萝卜素互补测定法和基因缺失分析，表明DbGGPS的编码区编码功能的GGPS。这为类胡萝卜素基因工程提供了新的基因来源。

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