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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Apolar Radical Initiated Conjugated Autoxidizable Triene (ApoCAT) Assay: Effects of Oxidant Locations on Antioxidant Capacities and Interactions
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Apolar Radical Initiated Conjugated Autoxidizable Triene (ApoCAT) Assay: Effects of Oxidant Locations on Antioxidant Capacities and Interactions

机译：非极性自由基引发的共轭可氧化三烯（ApoCAT）分析：氧化剂位置对抗氧化剂容量和相互作用的影响

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摘要

Development of an antioxidant assay explaining antioxidant behaviors in complex food systems has been a challenging topic for food scientists. This research aimed to investigate antioxidant capacities and interactions of selected synthetic antioxidants and commercial natural antioxidant extracts using the CAT assay and a newly developed ApoCAT assay, which used water- and lipid-soluble azo radical initiators, respectively. Results suggested that the higher the hydrophobicity of an antioxidant, the higher the antioxidant capacity of an antioxidant observed in the ApoCAT assay. The relationship between the two different assays was explained by the ratio between the ApoCAT and the CAT values. Interestingly, all lipophilic derivatives of the antioxidants exhibited higher ApoCAT/CAT ratios than their hydrophilic derivatives. In the case of the commercial food-grade antioxidants, green tea extract and mixed tocopherols showed a higher antioxidant capacity in the ApoCAT assay than in the CAT assay, while grape seed and rosemary extracts did not show significantly different changes in behaviors in both assays. The study on antioxidant interactions revealed that additive, synergistic, and antagonistic effects between hydrophilic antioxidants and natural extracts, and mixed tocopherols could be observed in both the CAT and the ApoCAT assays, depending on the combined ratios. In most cases, at a particular ratio, the synergistic effect reached the maximum level before suddenly dropping to additive and antagonistic effects in both assays.
机译：解释复杂食品系统中抗氧化剂行为的抗氧化剂测定方法的开发一直是食品科学家面临的挑战性课题。这项研究的目的是利用CAT分析法和新开发的ApoCAT分析法（分别使用水溶性和脂溶性偶氮自由基引发剂）研究选定的合成抗氧化剂和商业天然抗氧化剂提取物的抗氧化能力以及其相互作用。结果表明，抗氧化剂的疏水性越高，在ApoCAT分析中观察到的抗氧化剂的抗氧化剂能力就越高。两种不同测定之间的关系由ApoCAT和CAT值之比解释。有趣的是，所有抗氧化剂的亲脂性衍生物都比其亲水性衍生物具有更高的ApoCAT / CAT比。在商业食品级抗氧化剂的情况下，绿茶提取物和混合生育酚在ApoCAT分析中显示出比CAT分析更高的抗氧化能力，而葡萄籽和迷迭香提取物在两种分析中的行为均无明显差异。对抗氧化剂相互作用的研究表明，在CAT和ApoCAT分析中，取决于组合比例，可以观察到亲水性抗氧化剂和天然提取物以及混合生育酚之间的加性，协同和拮抗作用。在大多数情况下，在两种试验中，协同作用均以特定比例达到最大值，然后突然下降至加和拮抗作用。

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