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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.
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Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.

机译：马铃薯新品种糖生物碱生物合成相关基因的表征和转录​​特征

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Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to a-chaconine and a-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.
机译：在商业发布之前，必须评估新的马铃薯（Solanum tuberosum）品种的有毒化合物的含量，例如糖碱生物碱（GAs），这些化合物是强毒物。 GA生物合成通过胆固醇途径进行至α-查卡宁和α-茄碱。这项研究的目的是评估马铃薯块茎中总生物碱（TGA）含量与GAME，SGT1和SGT3基因表达之间的关系。通过HPLC MS测量TGA含量，并进行逆转录定量聚合酶链反应以确定GAME，SGT1和SGT3基因的相对表达。我们使用PlantPAN数据库搜索了转录起始位点的顺式元件。马铃薯块茎中TGA含量与GAME，SGT1和SGT3基因的相对表达之间存在相关性。推定的启动子区域显示与生物和非生物胁迫和光有关的几个顺式元件的存在。这些发现为理解马铃薯块茎中TGA的调控和变异提供了重要的一步。

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