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首页> 外文期刊>Cryobiology: International Journal of Low Temperature Biology and Medicine >Characteristics of actin fibers and ultrastructure of the contact regions involved in the separation of blastomeres of two-cell mouse embryos, frozen-thawed without the zona pellucida
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Characteristics of actin fibers and ultrastructure of the contact regions involved in the separation of blastomeres of two-cell mouse embryos, frozen-thawed without the zona pellucida

机译：肌动蛋白纤维的特性和接触区域的超微结构，涉及两细胞小鼠胚胎的卵裂球分离，无透明带的冷冻解冻

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摘要

Freezing of embryos deprived of the zona pellucida (ZP) decreases their survival rate immediately after thawing, and gives rise to the separation of their blastomeres in a high percentage of cases. We have studied the ultrastructure and the characteristics of actin fibers in the cell-to-cell contact region in mouse embryos frozen-thawed without the ZP at the two-cell stage. Our results indicate that most of the embryos that retain their blastomeres united after freezing and thawing show either the presence of a midbody, or a contact region with a close apposition of the plasma membranes but without an organized actin cortex in their contact region. Only a small percentage of embryos that retain their blastomeres united after freezing and thawing show a contact region with widely separated plasma membranes and an organized actin cytocortex.
机译：冻结了无透明带（Zona pellucida）（ZP）的胚胎后，其融化后的存活率立即降低，并且在很多情况下导致卵裂球分离。我们已经研究了在两细胞阶段无ZP冻融的小鼠胚胎细胞间接触区域中肌动蛋白纤维的超微结构和特征。我们的结果表明，冷冻和解冻后保留卵裂球统一的大多数胚胎显示中体的存在或质膜紧密并置的接触区，但在接触区中没有组织化的肌动蛋白皮层。冷冻和解冻后保留其卵裂球结合的胚胎中只有一小部分显示出与广泛分离的质膜和有组织的肌动蛋白细胞皮质的接触区域。

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