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首页> 外文期刊>Journal of Applied Polymer Science >Dye-Ligand Column Chromatography:Albumin Adsorption from Aqueous Media and Human Plasma with Dye-Affinity Microbeads
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Dye-Ligand Column Chromatography:Albumin Adsorption from Aqueous Media and Human Plasma with Dye-Affinity Microbeads

机译：染料-配体柱色谱：染料亲和微珠从水介质和人血浆中吸附白蛋白

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摘要

Cibacron Blue F3GA was covalently coupled with poly(ethylene glycol-dimethacrylate-2-hydroxyethylmethacrylate) [poly(EGDMA-HEMA)] microbeads via the nucleophilic substitution reaction between the chloride of itsa triazine ring and the hydroxyl groups of the HEMA molecules under alkaline conditions.The affinity sorbent carrying 16.5 #mu#mol Cibacron Blue F3GA/g polymer was then used for bovine serum albumin (BSA) adsorption from aqueous protein solutions and from human plasma in a packed-bed column.The BSA adsorption capacity of the microbeads decreased with an increase in the recirculation rate.High adsorption rates were observed at the beginning,then equilibrium was gradually achieved in about 60 min.The BSA concen-tration in the mobile phase was also effective on adsorption.BSA adsorption was firts increased with BSA concentration,then reached a plateau that was about 57.3 mg BSA/g.Higher BSA adsorption was observed at lower ionic strength.The maximum adsorption was observed at pH 5.0,which is the isoelectric pH of BSA.Higher human serum albumin adsorption was achieved from human plasma (109.6 mg HSA/g).High desorption ratios (over 94% of the adsorbed albumin) were achieved by using 1.0M NaSCN(pH 8.0) in 30 min.It was observed that albumin could be repeatedly adsorbed and desorbed without a significatn loss in adsorption capacity.
机译：烟碱蓝F3GA通过碱性条件下三嗪环的氯化物与HEMA分子羟基之间的亲核取代反应与聚乙二醇-二甲基丙烯酸-2-甲基丙烯酸羟乙酯[聚（EGDMA-HEMA）]微珠共价偶联载有16.5＃mu＃mol Cibacron Blue F3GA / g聚合物的亲和吸附剂随后被用于从蛋白质水溶液和填充床色谱柱中的人血浆中吸附牛血清白蛋白（BSA），微珠的BSA吸附能力降低在开始时观察到较高的吸附速率，然后在约60分钟内逐渐达到平衡。流动相中的BSA浓度也对吸附有效.BSA吸附随着BSA浓度的增加而增加然后达到约57.3 mg BSA / g的平稳期。在较低的离子强度下观察到较高的BSA吸附。在pH 5时观察到最大吸附。 0，这是BSA的等电点pH。从人血浆中获得更高的人血清白蛋白吸附（109.6 mg HSA / g）。通过使用1.0M NaSCN（pH 8.0）获得高解吸率（超过94％的吸附白蛋白） ）在30分钟内。观察到白蛋白可以重复吸附和解吸，而吸附能力没有明显损失。

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