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首页> 外文期刊>Journal of Applied Polymer Science >Pseudospecific magnetic affinity beads for immunoglobulin-g depletion from human serum
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Pseudospecific magnetic affinity beads for immunoglobulin-g depletion from human serum

机译：用于从人血清中清除免疫球蛋白-g的伪特异性磁亲和力珠

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摘要

Magnetic poly(ethylene glycol dimethacrylate-N-methacryloyl-(L)-histidine rnethyl ester) [m-poly(EGDMA-MAH)] beads were prepared by suspension polymerization for the affinity depletion of immunoglobulin-G (IgG) from human serum in a batch system. Elemental analysis of the magnetic beads for nitrogen was estimated as 70 mu mol MAH/g polymer. IgG adsorption onto the m-poly(EGDMA) was negligible. Higher adsorption value (LIP to 46.8 mg/g) was obtained in which the m-poly(EGDMA-MAH) beads were used. IgG adsorption capacity of the magnetic beads increased with an increase in the concentration of IgG. The maximum IgG adsorption was observed at pH 6.5 for MOPS buffer. IgG molecules could be repeatedly adsorbed and eluted with these adsorbents, without noticeable loss in their IgG adsorption capacity. Adsorption capacity decreased for both increasing salt concentration and temperature. In this study, we show that m-poly(EGDMA-MAH) beads (wherein IgG molecules bind directly with the matrix) can be used directly for affinity depletion without further modification. Higher adsorption value was obtained frorn human serum (up to 85.7 mg/g). The elution results demonstrated that the adsorption of IgG to the adsorbent was reversible. The depletion efficiencies for IgG were above 85% for all studied concentrations. Eluted portion was analyzed for testing the IgG removal efficiency by two dimensional gel electrophoresis. Eluted proteins include mainly IgG, and a small number of nonalbumin proteins such as apolipoprotein A1, serotransferrin, haptoglobulin, and alpha 1-antitrypsin. IgA was not identified in eluted fraction. (c) 2007 Wiley Periodicals.
机译：通过悬浮聚合制备磁性聚（乙二醇二甲基丙烯酸酯-N-甲基丙烯酰基-（L）-组氨酸甲基乙酯）[m-聚（EGDMA-MAH）]珠，以从人血清中亲和力去除免疫球蛋白-G（IgG）。批处理系统。氮对磁珠的元素分析估计为70μmol MAH / g聚合物。 IgG在m-poly（EGDMA）上的吸附率可以忽略不计。使用m-poly（EGDMA-MAH）磁珠可获得更高的吸附值（LIP至46.8 mg / g）。磁珠的IgG吸附能力随IgG浓度的增加而增加。对于MOPS缓冲液，在pH 6.5时观察到最大的IgG吸附。 IgG分子可以被这些吸附剂反复吸附和洗脱，而IgG吸附能力没有明显下降。随着盐浓度和温度的升高，吸附能力下降。在这项研究中，我们表明m-poly（EGDMA-MAH）珠（其中IgG分子直接与基质结合）可直接用于亲和力消耗，而无需进一步修饰。从人血清中获得更高的吸附值（高达85.7 mg / g）。洗脱结果表明，IgG对吸附剂的吸附是可逆的。在所有研究浓度下，IgG的耗竭效率均高于85％。通过二维凝胶电泳分析洗脱部分以测试IgG去除效率。洗脱的蛋白质主要包括IgG和少量非白蛋白蛋白质，例如载脂蛋白A1，血清转铁蛋白，触球蛋白和α1-抗胰蛋白酶。未在洗脱级分中鉴定出IgA。 （c）2007年Wiley期刊。

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