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首页> 外文期刊>Journal of Applied Polymer Science >Immobilization of myoglobin from horse skeletal muscle in hydrophilic polymer networks
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Immobilization of myoglobin from horse skeletal muscle in hydrophilic polymer networks

机译：亲水性聚合物网络中马骨骼肌肌红蛋白的固定化

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摘要

This work examines the immobilization of myoglobin from horse skeletal muscle in hydrophilic polymer networks. Due to specific changes in the spectroscopic properties of hemoproteins during ligand binding, they could be employed in optical sensing devices. Two immobilization techniques were considered: imbibition and entrapment. Anionic hydrogels composed of methacrylic acid (MAA), cationic hydrogels composed of dimethylamino ethyl methacrylate (DMAEM), and neutral hydrogels composed of poly(ethylene glycol) monomethyl ether monomethacrylate (PEGMA; molecular weight = 200, 400, or 1000), all crosslinked with poly(ethylene glycol) dimethacrylate (PEGDMA) (molecular weight = 200, 600, or 1000), were synthesized by free-radical solution polymerization. By the imbibition method, MAA-based hydrogels incorporated the highest amount of myoglobin in comparison with PEGMA or DMAEM polymers. The evaluation of the correlation length of the networks revealed that MAA hydrogels had the highest correlation length in comparison with PEGMA-containing matrices or DMAEM hydrogels. Release experiments from MAA hydrogels at pHs 5.8 and 7.0 showed that the solute-transport mechanism was a combination of Fickian and chain relaxation diffusion. Myoglobin-loaded MAA hydrogels retained their heme reactivity after the immobilization process. The release of myoglobin incorporated by entrapment in MAA-PEGDMA hydrogels was highly influenced by the chain relaxation process. The diffusion coefficients of myoglobin incorporated by entrapment into anionic hydrogels were 2 orders of magnitude smaller (similar to 10-13) than those for myoglobin incorporated by imbibition (10-11), both evaluated at pH 7.0. Substrate binding studies indicated that the protein biological activity was not compromised in those hydrogels loaded by the imbibition method, whereas prepolymeric solutions showed detrimental effects on protein stability. (C) 2007 Wiley Periodicals, Inc.
机译：这项工作检查了亲水聚合物网络中马骨骼肌肌红蛋白的固定。由于在配体结合过程中血蛋白的光谱特性发生了特定变化，因此它们可用于光学传感设备。考虑了两种固定技术：吸收和截留。由甲基丙烯酸（MAA）组成的阴离子水凝胶，由甲基丙烯酸二甲基氨基乙酯（DMAEM）组成的阳离子水凝胶和由聚（乙二醇）单甲基醚单甲基丙烯酸酯（PEGMA;分子量= 200、400或1000）组成的中性水凝胶均已交联通过自由基溶液聚合法合成了聚乙二醇（二甲基丙烯酸乙二醇酯）（PEGDMA）（分子量＝ 200、600或1000）。通过吸水法，与PEGMA或DMAEM聚合物相比，基于MAA的水凝胶掺入了最高量的肌红蛋白。网络相关长度的评估表明，与含PEGMA的基质或DMAEM水凝胶相比，MAA水凝胶具有最高的相关长度。 MAA水凝胶在pH值5.8和7.0的释放实验表明，溶质传输机制是Fickian和链弛豫扩散的组合。载有肌红蛋白的MAA水凝胶在固定过程后仍保持其血红素反应性。通过截留在MAA-PEGDMA水凝胶中并入的肌红蛋白的释放受到链松弛过程的高度影响。包埋到阴离子水凝胶中的肌红蛋白的扩散系数比通过吸收（10-11）的肌红蛋白的扩散系数小2个数量级（近似10-13），均在pH 7.0下评估。底物结合研究表明，在那些通过吸水法加载的水凝胶中，蛋白质的生物活性没有受到损害，而预聚物溶液则显示出对蛋白质稳定性的有害影响。 （C）2007 Wiley期刊公司

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