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首页> 外文期刊>Journal of Applied Polymer Science >Immobilized Metal Ions Cellophane-PGMA-Grafted Membranes for Affinity Separation of beta-Galactosidase Enzyme. I. Preparation and Characterization
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Immobilized Metal Ions Cellophane-PGMA-Grafted Membranes for Affinity Separation of beta-Galactosidase Enzyme. I. Preparation and Characterization

机译：固定化的金属离子玻璃纸-PGMA接枝的膜，用于β-半乳糖苷酶的亲和分离。一，制备与表征

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摘要

Immobilized Cu2+ ions affinity cellophane-poly(glycidyl methacrylate) (PGMA)-grafted membranes have been prepared through three steps. The first step was introducing of epoxy groups to its chemical Structure through grafting process with PGMA. Factors affecting the grafting process have been studied and grafting percentage (GP) up to 233%, has been obtained. The second step was converting the introduced epoxy groups to sulfonic ones. It was found that maximum amount of sulfonic groups (2.7 mmol/g) was obtained with minimum GP (46.08%). The third and last step was the immobilization of Cu2+ ions into sulfonated grafted membranes obtained from the previous step. Maximum amount of immobilized Cu2+ ions was found to be 60.9 ppm per grain of polymer. The verification of the grafting and sulfonation steps has been performed through characterization of the obtained membrane using FTIR, TGA, and EDAX analysis. Finally, Cu2+-immobilized membranes have been evaluated in separation of P-galactosidase (beta-Gal) enzyme from its mixture with bovine serum albumin (BSA) in different pH medium. Maximum protein adsorption, for both proteins, has been obtained at pH range 4-4.5; as 90 and 45%, for beta-Gal and BSA, respectively. The results showed high affinity toward beta-Gal separation although BSA concentration (0.5%) is 20-folds of beta-Gal (0.025%,). (C) 2008 Wiley, Periodicals, Inc. J Appl Polym Sci 111: 2647-2656, 2009
机译：通过三个步骤制备了固定化的Cu2 +离子亲和性玻璃纸-聚甲基丙烯酸缩水甘油酯（PGMA）接枝的膜。第一步是通过与PGMA的接枝过程将环氧基团引入其化学结构。已经研究了影响接枝过程的因素，并获得了高达233％的接枝率（GP）。第二步是将引入的环氧基转化为磺酸基。发现以最小的GP（46.08％）获得了最大量的磺酸基团（2.7mmol / g）。第三步也是最后一步是将Cu2 +离子固定到由上一步获得的磺化接枝膜中。发现固定的Cu 2+离子的最大量为每粒聚合物60.9 ppm。接枝和磺化步骤的验证已通过使用FTIR，TGA和EDAX分析表征获得的膜来进行。最后，在不同pH介质中，从其与牛血清白蛋白（BSA）的混合物中分离出P-半乳糖苷酶（β-Gal）的酶，已对固定Cu2 +的膜进行了评估。在4-4.5的pH范围内，两种蛋白质都获得了最大的蛋白质吸附。 β-Gal和BSA分别为90％和45％。结果显示，尽管BSA浓度（0.5％）是β-Gal的20倍（0.025％），但对β-Gal的分离具有很高的亲和力。 （C）2008 Wiley，Periodicals，Inc.J Appl Polym Sci 111：2647-2656，2009

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