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首页> 外文期刊>Journal of Applied Polymer Science >Magnetic Polymer Microspheres with Azidocarbonyl Groups: Synthesis, Characterization and Application in Protein Immobilization
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Magnetic Polymer Microspheres with Azidocarbonyl Groups: Synthesis, Characterization and Application in Protein Immobilization

机译：具有叠氮羰基的磁性聚合物微球：合成，表征和在蛋白质固定化中的应用。

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摘要

A novel magnetic polymer microsphere with amide groups and carboxyl groups was synthesized and reported here. The azidocarbonyl groups were derived from amide groups and linked to the proteins to investigate their immobilization capacity. The morphology, size, functional groups and magnetic properties of magnetic microspheres were characterized by optical microscopy, particle size analyzer, atom force microscopy, magnetic force microscopy, fourier transform infrared spectrometer, vibrating-sample magnetometer and thermal gravimetric analysis. The results indicated that the magnetic polymer microspheres had a well spherical shape with the size ranging from 1 to 10 lm, highly reactive functional groups, superparamagnetism and strong magnetic responsibility with saturation magnetization of 18.443 emu/g and Fe3O4 content around 21%. The immobilization capacity (g) was over 70%. The novel azidocarbonyl magnetic polymer microspheres showed potentials to be a good magnetic support and promising applications in bioseparation and biomedical fields.
机译：合成并报道了具有酰胺基和羧基的新型磁性聚合物微球。叠氮基羰基衍生自酰胺基并与蛋白质连接以研究其固定能力。通过光学显微镜，粒度分析仪，原子力显微镜，磁力显微镜，傅立叶变换红外光谱仪，振动样品磁力计和热重分析法对磁性微球的形貌，尺寸，官能团和磁性进行了表征。结果表明，磁性聚合物微球具有良好的球形形状，尺寸范围为1至10 lm，具有高反应活性的官能团，超顺磁性和强磁性，饱和磁化强度为18.443 emu / g，Fe3O4含量约为21％。固定容量（g）超过70％。新型叠氮羰基磁性聚合物微球显示出潜在的良好磁支持和在生物分离和生物医学领域的应用前景。

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