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Synthesis and Antioxidant Activity Evaluation of Novel Broom and Cotton Fibers Derivatives

机译:新型扫帚和棉纤维衍生物的合成及抗氧化活性评估

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Novel antioxidant biopolymers composed by broom or cotton fibers, and trans-ferulic acid, alpha-lipoic acid, and alpha-tocopherol, were prepared. The acids were directly linked to fibers microfibrils hydroxylic groups using N,N-dimethylacetamide/lithium chloride (DMAc/LiCl) as solvent with dicyclohexylcarbodiimide (DCC) and N,N-dimethylaminopyridine (DMAP). Broom or cotton tocopherulate were prepared in the same conditions by using carboxylated fibers. Ester linkages were confirmed by using Fourier transformed infrared spectroscopy; biopolymers substitution degrees (DS) were determined by volumetric analysis. Antioxidant activity in inhibiting lipid peroxidation, in rat-liver microsomal membranes, induced in vitro by two different sources of free radicals, 2,2'-azobis (2-amidinopropane) (AAPH) and tert-butyl hydroperoxide (tert-BOOH), was evaluated. Finally, biocompatibility of these biomaterials was also tested by controlling glutathione-S-transferase (GST) basal concentrations. Our results strongly suggested that the better derivatives were the ferulate ones. However, all obtained biomaterials, showing excellent antioxidant properties and biocompatibility, could be successfully applied in biomedical and in packaging areas.
机译:制备了由扫帚或棉纤维,反式阿魏酸,α-硫辛酸和α-生育酚组成的新型抗氧化剂生物聚合物。使用N,N-二甲基乙酰胺/氯化锂(DMAc / LiCl)作为溶剂,并用二环己基碳二亚胺(DCC)和N,N-二甲基氨基吡啶(DMAP),将酸直接连接到纤维微纤维的羟基上。用羧化纤维在相同条件下制备扫帚或棉络合物。通过使用傅立叶变换红外光谱法确认酯键。通过体积分析确定生物聚合物的取代度(DS)。两种不同来源的自由基在体外诱导的大鼠肝脏微粒体膜中抗脂质过氧化的抗氧化剂活性:2,2'-偶氮双(2-ami基丙烷)(AAPH)和叔丁基过氧化氢(tert-BOOH),被评估。最后,还通过控制谷胱甘肽-S-转移酶(GST)的基础浓度来测试这些生物材料的生物相容性。我们的结果强烈表明,较好的衍生物是阿魏酸。但是,所有获得的具有优异抗氧化性能和生物相容性的生物材料都可以成功地应用于生物医学和包装领域。

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