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首页> 外文期刊>Journal of Applied Polymer Science >Preparation and characterization of porous poly(vinyl ester) resin monoliths as separation media
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Preparation and characterization of porous poly(vinyl ester) resin monoliths as separation media

机译：分离介质为多孔聚乙烯酯树脂整体料的制备与表征

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摘要

Porous poly(vinyl ester) resin monolithic supports were first prepared by radical polymerization of the continuous phase of oil in water high-internal-phase emulsions. Vinyl ester (VE) resin was used as the monomer, ethylene glycol dimethacrylate was used as a crosslinker, and poloxamer 127 was used as the emulsifier in the emulsion polymerization. The prepared columns were evaluated by scanning electron microscopy, mercury intrusion porosimetry, and Fourier transform infrared spectroscopy to observe the morphological characteristics and confirm the absorbance based on the VE resin polymer. The obtained monolith showed not only higher column permeability but also lower back pressure and higher column efficiency. To investigate the absorption performance of the monolithic column, a maximum loading capacity experiment was also applied with lysozyme (Lys), and the results show that the maximum adsorption of the poly(vinyl ester) resin monolith was 1.579 mg/g. Moreover, the capabilities of separation on this column in conjunction with high-performance liquid chromatography were investigated. Immunoglobulin could be separated from human plasma and chicken egg yolk with high resolution within 4 min. Additionally, fast separation of two mode proteins (interleukin-18 and Lys) was achieved on the monolith within 2 min at the rate of 1445 cm/h, which demonstrated the potential of the poly(vinyl ester) resin monolith for the fast separation of proteins.
机译：首先通过水包油高内相乳液连续相的自由基聚合，制备多孔聚乙烯基树脂整体式载体。在乳液聚合中，使用乙烯基酯（VE）树脂作为单体，使用乙二醇二甲基丙烯酸酯作为交联剂，并使用泊洛沙姆127作为乳化剂。通过扫描电子显微镜，压汞法和傅里叶变换红外光谱法对所制备的色谱柱进行评估，以观察其形态特征并确认基于VE树脂聚合物的吸光度。所获得的整料不仅显示出更高的柱渗透性，而且还显示出更低的背压和更高的柱效。为了研究整体柱的吸收性能，还对溶菌酶（Lys）进行了最大负载量实验，结果表明，聚（乙烯基酯）树脂整体柱的最大吸附量为1.579 mg / g。此外，还结合高效液相色谱法研究了该色谱柱的分离能力。免疫球蛋白可以在4分钟内从人体血浆和蛋黄中分离出来。此外，在2分钟内以1445 cm / h的速度在整料上实现了两种模式蛋白（白介素18和Lys）的快速分离，这证明了聚（乙烯基酯）树脂整料可快速分离出两种模式蛋白。蛋白质。

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