• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Applied Polymer Science >Polyacrylamide-grafted carboxymethyl cellulose: Smart pH-sensitive hydrogel for protein concentration
【24h】

Polyacrylamide-grafted carboxymethyl cellulose: Smart pH-sensitive hydrogel for protein concentration

机译：聚丙烯酰胺接枝的羧甲基纤维素：用于蛋白质浓缩的智能pH敏感水凝胶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel application, utilizing polyacrylamide-g-carboxymethyl cellulose (CMC-g-PAM) in concentrating dilute solutions of Bovine serum albumin (BSA), was investigated. The grafting reaction parameters were investigated and the hydrogel smartness was verified. FT-IR proved that the grafting reaction occurred between the hydroxyl group located in anhydroglucose C_2 position of CMC and the π-bond of PAM and SEM confirmed a changed morphology to a fibrillar structure. The pH sensitivity was proved; as the grafted polymer attained its maximum swelling at pH 7.2 while the minimum swelling was observed under acidic conditions (pH 1-3). The rate of water uptake in the grafted polymer hydrogel was higher than that of the homopolymer hydrogel and the swelling behaviors of both hydrogels obeyed second-order kinetics. The tested hydrogel showed a high potency towards concentrating BSA solutions with a concentration factor of 1 to 4.5 times and recovery of 60-90%. The concentration factor increased linearly with increasing both the polymer concentration and the process time and decreased with the increase in the protein concentration. The grafted polymer had stable efficiency in the concentration process for 20 cycles. The obtained results have recommended the employment of the prepared CMC-g-PAM hydrogel in the down stream protein concentration process in the industrial scale.
机译：研究了一种利用聚丙烯酰胺-g-羧甲基纤维素（CMC-g-PAM）浓缩牛血清白蛋白（BSA）稀溶液的新应用。研究了接枝反应参数并验证了水凝胶的灵敏性。 FT-IR证实了接枝反应发生在CMC的脱水葡萄糖C_2位与PAM的π键之间，并且SEM证实了纤维状结构的形态发生了变化。证明了pH敏感性;因为接枝聚合物在pH 7.2时达到最大溶胀，而在酸性条件下（pH 1-3）则观察到最小溶胀。接枝的聚合物水凝胶的吸水率高于均聚物的水凝胶，两种水凝胶的溶胀行为均服从二级动力学。所测试的水凝胶显示出浓缩BSA溶液的高效力，浓缩系数为1至4.5倍，回收率为60-90％。浓度因子随聚合物浓度和处理时间的增加而线性增加，而随蛋白质浓度的增加而减小。接枝的聚合物在浓缩过程中具有20个循环的稳定效率。获得的结果建议在工业规模的下游蛋白质浓缩过程中使用制备的CMC-g-PAM水凝胶。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号