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首页> 外文期刊>Journal of Applied Polymer Science >Solid-phase extraction of S-(-)-amlodipine from plasma with a uniformly sized molecularly imprinted polymer
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Solid-phase extraction of S-(-)-amlodipine from plasma with a uniformly sized molecularly imprinted polymer

机译：均相分子印迹聚合物从血浆中固相萃取S-（-）-氨氯地平

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摘要

To specifically extract S-(-)-amlodipine from plasma, uniformly sized molecularly imprinted polymers (MIPs) for S-(-)-amlodipine were prepared in an aqueous system by multistep swelling and polymerization with methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a crosslinker, and toluene as a porogen. Scanning electron microscopy was used to identify the structural features of the obtained polymers. ~1H-NMR and high-performance liquid chromatographic analysis were performed to explore the possible recognition mechanism. The results reveal that spherical polymer beads with uniform size and good monodispersity were obtained, and the MIPs showed specific recognition ability for the template molecule. The ionic hydrophobic and hydrogen-bonding interactions were inferred to play an important role in the recognition mechanism. The results indicate that the MIPs could be used as a solid-phase extraction sorbent for the concentration and purification of S-(-)-amlodipine from plasma with a high efficiency. The linear range was 0.25-8.00 μg/mL with a correlation coefficient of 0.9948. The average recovery was 98.3% with relative standard deviation (RSD) less than 9.1%.
机译：为了从血浆中特异性提取S-（-）-氨氯地平，通过以甲基丙烯酸为功能单体，乙二醇进行多步溶胀和聚合，在水性体系中制备了S-（-）-氨氯地平的均一大小的分子印迹聚合物（MIP）。二甲基丙烯酸酯作为交联剂，甲苯作为成孔剂。扫描电子显微镜用于鉴定所得聚合物的结构特征。进行了〜1H-NMR和高效液相色谱分析，以探讨可能的识别机理。结果表明，获得了球形聚合物珠，具有均匀的尺寸和良好的单分散性，并且MIPs显示出对模板分子的特异性识别能力。推测离子疏水和氢键相互作用在识别机理中起重要作用。结果表明，MIPs可以作为固相萃取吸附剂用于高效地从血浆中浓缩和纯化S-（-）-氨氯地平。线性范围为0.25-8.00μg/ mL，相关系数为0.9948。平均回收率为98.3％，相对标准偏差（RSD）小于9.1％。

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