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首页> 外文期刊>Journal of Applied Polymer Science >A novel water-soluble polythiophene derivatives based fluorescence 'turn-on' method for protein determination
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A novel water-soluble polythiophene derivatives based fluorescence 'turn-on' method for protein determination

机译：一种基于水溶性聚噻吩衍生物的新型荧光“开启”蛋白质测定方法

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A lable-free, simple, and sensitive fluorescence "turn-on" approach is designed to rapidly detect protein using a conjugated polythiophene derivative (PDPMT-Cl). The fluorescence of PDPMT-Cl solution can be efficiently quenched by PtCl42- ions. Upon adding trypsin to the (bovine serum albumin, BSA) PDPMT-Cl-PtCl42- solution, the BSA is cleaved into amino acid or peptide fragments, which are stronger PtCl42- ions chelators to form more stable complexes with PtCl42- ions. Thus, the PtCl42- ion is displaced from PDPMT-Cl and the fluorescence of PDPMT-Cl is recovered. By triggering the "turn-on" signal of PDPMT-Cl, it is successful to detect the protein in real time. "Turn-on" response as readout signal is able to effectively reduce background noise and increase detection sensitivity. This method offers good selectivity for detecting protein in the presence of other common amino acids and metal ions. Under optimized conditions, the concentration of BSA in the range of 0.0004-1.75 mg/mL exhibits a linear relationship with the relative fluorescence intensity, and the correlation coefficient is 0.9997. The limit of detection is 4.47 × 10-4 mg/mL. The system is successfully applied for detecting protein in milk and egg. Due to the simplicity, sensitivity, and rapid response, this assay shows great potential for protein detection in the future.
机译：一种无标签，简单且敏感的荧光“开启”方法被设计为使用共轭聚噻吩衍生物（PDPMT-Cl）快速检测蛋白质。 PDPMT-Cl溶液的荧光可以被PtCl42-离子有效地猝灭。将胰蛋白酶添加到（牛血清白蛋白，BSA）PDPMT-Cl-PtCl42-溶液中后，BSA被切割成氨基酸或肽片段，它们是更强的PtCl42-离子螯合剂，与PtCl42-离子形成更稳定的复合物。因此，PtCl 4-2离子从PDPMT-Cl置换，并且PDPMT-Cl的荧光被回收。通过触发PDPMT-Cl的“开启”信号，可以成功实时检测蛋白质。作为读取信号的“接通”响应能够有效降低背景噪声并提高检测灵敏度。该方法为在存在其他常见氨基酸和金属离子的情况下检测蛋白质提供了良好的选择性。在最佳条件下，BSA的浓度在0.0004-1.75 mg / mL范围内与相对荧光强度呈线性关系，相关系数为0.9997。检测限为4.47×10-4 mg / mL。该系统已成功应用于牛奶和鸡蛋中蛋白质的检测。由于其简单性，灵敏性和快速响应性，该测定法在未来显示出巨大的蛋白质检测潜力。

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