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首页> 外文期刊>Journal of Applied Polymer Science >Extrusion-based differences in two types of nylon 6 capillary-channeled polymer (C-CP) fiber stationary phases as applied to the separation of proteins via ion exchange chromatography
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Extrusion-based differences in two types of nylon 6 capillary-channeled polymer (C-CP) fiber stationary phases as applied to the separation of proteins via ion exchange chromatography

机译：两种尼龙6毛细管聚合物（C-CP）纤维固定相中基于挤出的差异，这些差异适用于通过离子交换色谱法分离蛋白质

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摘要

Nylon 6 capillary-channeled polymer (C-CP) fibers used as the stationary phase in high-performance liquid chromatography protein separations have been investigated. A difference in chromatographic behavior between two (presumably identical) nylon 6 C-CP fibers, designated simply as nylon 6A and nylon 6B, reveals unknown differences in their chemical and physical nature as proteins are less retained on the latter. The possible differences are evaluated through several test methods, including Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), mechanical testing, MALDI-TOF mass spectrometry, an organic reaction with ninhydrin, and dilute solution viscometry. Nylon 6B is shown to be a larger perimeter, stronger fiber, yet in a 200 mm, 0.8 mm i.d. column having an interstitial fraction of ～0.6, it has an available surface area of ～189 cm~2, while nylon 6A is almost double that at ～374 cm~2. It is revealed that when in amorphous form, the base polymers have very comparable FTIR and DSC characteristics. When in their solid, extruded fiber form there are significant differences found in crystallinity, tensile strength, molecular weight distribution, and end group density. Nylon 6A is a lower molecular weight fiber, which results in ～1.3× more amine end groups than nylon 6B. It is the end group density that ultimately determines the separation qualities. These results imply that both a difference in base nylon 6 molecular weight and also the fiber extrusion process have a significant influence on the performance of nylon 6 C-CP fiber separation of proteins via ion exchange chromatography.
机译：已经研究了在高性能液相色谱蛋白质分离中用作固定相的尼龙6毛细管聚合物（C-CP）纤维。两种（大概是相同的）尼龙6 C-CP纤维之间的色谱行为差异（简称为尼龙6A和尼龙6B）显示出化学和物理性质方面的未知差异，因为蛋白质在后者上的保留较少。可能的差异通过几种测试方法进行评估，包括傅立叶变换红外光谱（FTIR），差示扫描量热法（DSC），机械测试，MALDI-TOF质谱，茚三酮的有机反应以及稀溶液粘度法。尼龙6B被证明是更大的周长，更坚固的纤维，但内径为200 mm，0.8 mm。间隙分数为〜0.6的圆柱，其有效表面积为〜189 cm〜2，而尼龙6A几乎是在374 cm〜2时的两倍。揭示了当处于无定形形式时，基础聚合物具有非常可比的FTIR和DSC特性。当以其固体，挤出的纤维形式存在时，在结晶度，拉伸强度，分子量分布和端基密度方面存在显着差异。尼龙6A是一种较低分子量的纤维，比尼龙6B的胺端基多出约1.3倍。最终基团的密度最终决定了分离质量。这些结果表明，基础尼龙6分子量的差异以及纤维挤出工艺均对通过离子交换色谱分离蛋白质的尼龙6 C-CP纤维的性能产生重大影响。

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