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首页> 外文期刊>Journal of Applied Polymer Science >Effective immobilization of lipase onto a porous gelatin-co-Poly(vinyl alcohol) copolymer and evaluation of its hydrolytic properties
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Effective immobilization of lipase onto a porous gelatin-co-Poly(vinyl alcohol) copolymer and evaluation of its hydrolytic properties

机译：将脂肪酶有效地固定在多孔明胶-共-聚乙烯醇共聚物上并评估其水解性能

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摘要

Crosslinked copolymers of gelatin and poly(vinyl alcohol) (PVA) with excellent water absorption and water retention abilities were successfully synthesized using ~(60)Co γ radiation. Ammonium persulfate (APS), as a water-soluble initiator and sodium bicarbonate (NaHCO_3) as a foaming agent were used. The best synthesis conditions were evaluated with regard to the maximum percentage of swelling as a function of the APS concentration, NaHCO_3 concentration, amount of water, and reaction time. The maximum swelling percentage (1694.59%) of the copolymer gelatin-co-PVA, was obtained at the optimum parameters [APS] = 2.92 × 10~(-1) mol/L, [NaHCO_3] = 7.94 × 10~(-2) mol/L, and 1.5 mL of water with 31.104 kGy of the γ radiation dose. The copolymer was characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. The SEM analysis showed a highly nanoporous and cellular structure of the copolymer. The copolymer was used as a support for lipase immobilization. The optimization of the reaction conditions, including the pH and temperature for immobilization, on the basis of the hydrolysis of p-nitrophenyl palmitate, was carried out. An excellent efficiency for protein loading (70%) at pH 8.5 by the copolymer was observed. The results observed during the evaluation of the hydrolytic properties showed excellent activity of the bound lipase. The porous gelatin-co-PVA bound lipase was found to be stable at 75°C and pH 8.5; it displayed 2.326 ± 0.005 U/g of lipase activity. The stability and activity of the copolymer-bound lipase were also studied as a function of the time at 75°C, and the biocatalyst was found to be stable and active up to 1 h, beyond which the activity decreased.
机译：利用〜（60）Coγ射线成功地合成了具有优良的吸水性和保水性的明胶和聚乙烯醇（PVA）的交联共聚物。使用过硫酸铵（APS）作为水溶性引发剂，并使用碳酸氢钠（NaHCO_3）作为发泡剂。关于溶胀的最大百分比与APS浓度，NaHCO 3浓度，水量和反应时间的关系，评估了最佳合成条件。在最佳参数[APS] = 2.92×10〜（-1）mol / L，[NaHCO_3] = 7.94×10〜（-2）的条件下，获得了明胶-co-PVA共聚物的最大溶胀率（1694.59％）。摩尔/升）和1.5毫升水，以及31.104 kGy的γ辐射剂量。通过傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）方法对共聚物进行了表征。 SEM分析表明该共聚物具有高度的纳米孔和孔结构。将该共聚物用作脂肪酶固定化的载体。基于对硝基苯基棕榈酸酯的水解，进行了反应条件的最优化，包括用于固定的pH和温度。观察到在共聚物的pH 8.5下，蛋白质负载的效率极高（70％）。在评估水解性能的过程中观察到的结果表明结合的脂肪酶具有出色的活性。发现多孔明胶-co-PVA结合的脂肪酶在75℃和pH 8.5下是稳定的。它显示出2.326±0.005 U / g的脂肪酶活性。还研究了与共聚物结合的脂肪酶的稳定性和活性随时间在75°C的变化，发现生物催化剂在1小时内是稳定且有活性的，超过此时间活性降低。

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