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首页> 外文期刊>Journal of Applied Polymer Science >Structural and Functional Changes of Silk Fibroin Scaffold Due to Hydrolytic Degradation
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Structural and Functional Changes of Silk Fibroin Scaffold Due to Hydrolytic Degradation

机译：丝素蛋白支架的结构和功能变化由于水解降解。

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In this study, hydrolytic degradation of silk fibroin (SF) in Phosphate buffer saline (PBS) after 12 weeks incubation was investigated. Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and X-ray diffraction (XRD) patterns have confirmed the transition from crystalline β-sheet to random coil in treated SF. A decrease in adhesion force and surface Young's modulus were observed using atomic force microscopy (AFM). Structural changes were further confirmed using scanning electron microscopy (SEM). Biocompatibility and alkaline phosphatase production of osteoblast cells were decreased significantly in treated SF scaffold. Moreover, a significant decrease in mRNA level of collagen type I and osteopontin compared with fresh SF scaffold was observed. Finally, structural and biological characteristics of SF scaffold could alter in PBS.
机译：在这项研究中，研究了在孵育12周后在磷酸盐缓冲盐水（PBS）中丝素蛋白（SF）的水解降解。傅里叶变换红外光谱（FTIR），拉曼光谱和X射线衍射（XRD）模式已经证实了处理过的SF中从结晶β-片层到无规卷曲的转变。使用原子力显微镜（AFM）观察到粘合力和表面杨氏模量降低。使用扫描电子显微镜（SEM）进一步确认了结构变化。在处理过的SF支架中，成骨细胞的生物相容性和碱性磷酸酶产生显着降低。而且，与新鲜的SF支架相比，观察到I型胶原和骨桥蛋白的mRNA水平显着降低。最后，SF支架的结构和生物学特性可能在PBS中发生变化。

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