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首页> 外文期刊>Journal of Applied Polymer Science >Analysis of the effects of electrostatic interactions on protein transport through zwitterionic ultrafiltration membranes using protein charge ladders
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Analysis of the effects of electrostatic interactions on protein transport through zwitterionic ultrafiltration membranes using protein charge ladders

机译：使用蛋白质电荷梯分析静电相互作用对两性离子超滤膜转运蛋白质的影响

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摘要

A large number of studies have shown that zwitterionic ultrafiltration membranes have very low protein fouling due to the strongly hydrated structure of the zwitterionic modification. However, there is much less known about the effects of the zwitterionic functionality on the electrostatic interactions governing protein transport through these membranes. The objective of this work was to use protein charge ladders to evaluate the effects of electrostatic interactions on protein transport through cellulosic ultrafiltration membranes modified with a zwitterionic functionality. Data were obtained using protein charge ladders formed by reacting lysozyme and -lactalbumin with acetic anhydride to generate a series of protein derivatives (ladders) differing by single charge groups but with essentially identical size. Protein retention was greater for the more positively charged elements within the charge ladder, consistent with a weak electrostatic repulsion from the zwitterionic membrane which had a small positive charge at pH 7. Data for all elements of the protein charge ladder (both positively and negatively charged) were in excellent agreement with calculations of a theoretical model based on the partitioning of a charged sphere (protein) in a charged cylinder. The results demonstrate the potential of using zwitterionic membranes for enhanced ultrafiltration with high selectivity and minimal fouling. (c) 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41540.
机译：大量研究表明，两性离子超滤膜由于两性离子修饰的强水合结构而具有极低的蛋白质结垢。但是，两性离子官能团对控制蛋白质通过这些膜运输的静电相互作用的影响知之甚少。这项工作的目的是使用蛋白质电荷阶梯来评估静电相互作用对通过两性离子官能团修饰的纤维素超滤膜转运蛋白质的影响。使用溶菌酶和-乳清蛋白与乙酸酐反应生成一系列蛋白质衍生物（梯形图）而得到的数据，这些蛋白质梯形图通过单电荷基团不同但大小基本相同，从而形成一系列蛋白质衍生物（梯形图）。电荷梯中带更多正电荷的元素的蛋白质保留更大，这与两性离子膜的弱静电排斥作用（在pH 7时具有小的正电荷）相符。蛋白质梯形图的所有元素（带正电荷和带负电荷的数据） ）与基于带电圆柱体中带电球体（蛋白质）分配的理论模型的计算非常吻合。结果表明，使用两性离子膜具有增强的超滤性能，高选择性和最小污垢的潜力。 （c）2014 Wiley Periodicals，Inc. J. Appl。 Polym。科学2015，132，41540。

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