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首页> 外文期刊>Journal of Applied Polymer Science >Effect of Electrostatic Functional Monomers on Lysozyme Recognition by Molecularly Imprinted Hydrogel
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Effect of Electrostatic Functional Monomers on Lysozyme Recognition by Molecularly Imprinted Hydrogel

机译：静电功能单体对分子印迹水凝胶对溶菌酶识别的影响

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Electrostatic functional monomers (EFMs) play an important role in noncovalent molecular imprinting due to their formed complexes with the complementary segment(s) of the template molecule. In this work using UV difference spectroscopy, interaction saturations of methacrylic-acid and 2-dimethyl-amino-ethyl-methacrylate in complex formation with lysozyme (Lyz) surface was found to occur at molar ratios to Lyz of 400 and 100, respectively. Based on these results and the estimated free to total EFMs ratios, four sets of imprintedonimprinted hydrogel samples were synthesized alongside the two sets based on lysozyme surface charges and equal EFMs. Comparisons showed that the highest absorption capacity of 59 mg/g and imprinting effect of 1.58 correspond to samples with EFM/Lyz ratios at saturation and minimum free to total EFM/Lyz ratios, respectively. Minimization of free monomers is hence important in recognition of proteins by avoiding the nonspecific binding. This can have generic application for specific separation of other macromolecules. (c) 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41366.
机译：静电功能单体（EFM）由于与模板分子的互补链段形成复合物，因此在非共价分子印迹中起重要作用。在这项使用紫外差光谱法的工作中，发现与溶菌酶（Lyz）表面形成复合物的甲基丙烯酸和2-二甲基-氨基-乙基-甲基丙烯酸酯的相互作用饱和分别发生在与Lyz的摩尔比为400和100的情况下。根据这些结果和估计的自由/总EFM比率，基于溶菌酶表面电荷和相等的EFM，合成了两组印迹/未印迹水凝胶样品以及两组样品。比较表明，最高吸收容量为59 mg / g，印迹效果为1.58，分别对应于饱和状态下具有EFM / Lyz比的样品，而自由度与总EFM / Lyz之比最小。因此，通过避免非特异性结合，最小化游离单体对于识别蛋白质非常重要。这可以用于其他大分子的特异性分离。 （c）2014 Wiley Periodicals，Inc. J. Appl。 Polym。科学2015，132，41366。

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