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Production of silk fibroin nanoparticles using ionic liquids and high-power ultrasounds

机译:使用离子液体和大功率超声波生产丝素蛋白纳米颗粒

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Biopolymeric nanoparticles have attracted great research interest in the last few years due to their multiple applications. This article describes how high-power ultrasounds are capable of enhancing the dissolution process of silk proteins in ionic liquids (ILs) and how silk fibroin nanoparticles (SFNs) can be obtained directly from the silk/ionic liquid solution (SIL) by rapid desolvation in polar organic solvents. The silk fibroin integrity is highly preserved during the dissolution process, as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the SIL. These regenerated SFNs are insoluble in water and other common organic solvents and are indistinguishable from the classical SFNs with respect to their diameter (180 +/- 5 nm), Zeta potential (-25 +/- 3 mV), high degree of -sheet and low cytotoxicity. Large amounts of silk can be turned into biomaterials directly from the SIL solution for use in a wide range of applications, while the ILs can be recovered from the coagulant solution under reduced pressure and reused without loss of their solvent properties. (c) 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41702.
机译:近年来,生物聚合物纳米颗粒由于其多种用途而引起了极大的研究兴趣。本文介绍了高功率超声如何能够增强丝蛋白在离子液体(ILs)中的溶解过程,以及如何通过快速脱溶剂从丝/离子液体溶液(SIL)直接获得丝素蛋白纳米颗粒(SFN)。极性有机溶剂。 SIL的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)证实了丝蛋白的完整性在溶解过程中得到了高度保留。这些再生的SFN不溶于水和其他常见的有机溶剂,就其直径(180 +/- 5 nm),Zeta电位(-25 +/- 3 mV),高薄片强度而言,与传统SFN没有区别。和低细胞毒性。可以将大量的丝直接从SIL溶液直接转化为生物材料,以用于广泛的应用,而IL可以在减压下从凝结剂溶液中回收并重新使用,而不会损失其溶剂性能。 (c)2014 Wiley Periodicals,Inc. J. Appl。 Polym。科学2015,132,41702。

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