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首页> 外文期刊>Journal of Applied Polymer Science >Spectroscopic Analysis and Catalytic Application of Biopolymer Capped Silver Nanoparticle, an Effective Antimicrobial Agent
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Spectroscopic Analysis and Catalytic Application of Biopolymer Capped Silver Nanoparticle, an Effective Antimicrobial Agent

机译：一种有效的抗菌剂生物聚合物包覆的银纳米粒子的光谱分析和催化应用

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摘要

Microbial reduction of silver ion (conc. 1 mM AgNO3) was performed by Alkaliphilus oremlandii strain ohILAs in an alkaline pH 10. The synthesized silver nanoparticle was stabilized by poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer which was also synthesized by the microbial culture of Alkaliphilus oremlandii strain ohILAs at pH8. The particle size and shape of the silver nanoparticles was studied by dynamic light scattering and under a transmission electron microscope and it was found that the particle size of polymer stabilized colloidal silver was comparatively lower (22-43nm) than that for the unstabilized one (63-93 nm). The stabilization of nanoparticles in polymer dispersed medium after around 60 days was confirmed from analysis of UV-visible spectroscopy and scanning electron microscopy. The crystalline peaks as recorded with X-ray's diffraction were observed at 2 values of 38 degrees and 43 degrees, indicating the fcc crystalline structure of the silver nanoparticle. The antimicrobial activity of silver nanoparticles on gram-negative bacteria strain (Escherichia coli XL1B) and gram-positive strain (Lysinibacillus fusiformis) showed better performance by the solution of polymer stabilized nanoparticle than that for the non polymer stabilized one. The reduction of nitro group in p-nitrophenol to p-aminophenol was observed from the analysis of UV-Visible spectroscopy in which, the shifting of absorption peak at 400 to 295 nm and the simultaneous regeneration of light brown color ((max) 410 nm) of silver nanoparticles confirmed the catalytic activity of silver nanomaterials. (c) 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41495.
机译：在碱性pH 10下，由碱性嗜碱菌ohILAs菌株对银离子（浓度为1 mM AgNO3）进行微生物还原。合成的银纳米颗粒由聚（3-羟基丁酸酯-co-3-羟基戊酸酯）生物聚合物稳定化，该聚合物也通过pH为8时，嗜碱性产碱杆菌ohILAs菌株的微生物培养。通过动态光散射和透射电子显微镜研究了银纳米粒子的粒径和形状，发现聚合物稳定的胶态银的粒径（22-43nm）比未稳定的胶态银（63-63nm）要低。 -93 nm）。通过UV-可见光谱和扫描电子显微镜的分析证实了约60天后纳米颗粒在聚合物分散介质中的稳定性。用X射线衍射记录的结晶峰在38度和43度的2个值处观察到，表明银纳米粒子的fcc晶体结构。纳米银溶液对革兰氏阴性菌菌株（大肠埃希氏菌XL1B）和革兰氏阳性菌株（梭状芽孢杆菌）的抗菌活性均优于非高分子稳定化纳米粒。从紫外-可见光谱分析可观察到对硝基苯酚中的硝基还原为对氨基苯酚，其中吸收峰在400至295 nm处移动，并同时再生浅棕色（（最大）410 nm银纳米颗粒的）证实了银纳米材料的催化活性。 （c）2014 Wiley Periodicals，Inc. J. Appl。 Polym。科学2015，132，41495。

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