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首页> 外文期刊>Journal of Applied Polymer Science >Application of a C6-OH of Chitosan Immobilized Cyclodextrin Derivates on an Electrochemical H2O2 Biosensor
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Application of a C6-OH of Chitosan Immobilized Cyclodextrin Derivates on an Electrochemical H2O2 Biosensor

机译：壳聚糖固定化环糊精衍生物的C6-OH在电化学H2O2生物传感器上的应用

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Biosensor detecting techniques have attracted much attention in the content determination of H2O2, which has been used illegally as a food additive. An electrochemical biosensing membrane for the detection of H2O2 was developed with C6-OH of chitosan immobilized cyclodextrin derivates (6-CD-CTS), which possessed a high cyclodextrin loading capacity (2.12 x 10(-4) mol/g), as the carrier. The biosensor was prepared through the inclusion of ferrocene as the electron mediator in a hydrophobic cavity of cyclodextrin and crosslinking catalase (CAT) to 2-NH2 of 6-CD-CTS. The ferrocene-included complex was evaluated by ultraviolet-visible spectrophotometry and thermogravimetric analysis. Its electrochemical behavior was also studied. The impact of the reaction conditions on the CAT immobilization capacity was evaluated. When previous membrane was used to detect the concentration of H2O2 (C-H2O2), we found that the catalysis of CAT and the signal amplification of ferrocene had a major impact on the cyclic voltammograms. The optimal working pH of the modified electrode was 7.0. The peak current (I) had a linear relationship with the H2O2 concentration (CH2O2) in the range 1.0 x 10(-4) to 1.0 x 10(-3) mol/L. The linear regression equation was I=0.00475C(H2O2)-0.03025. The detection limit was 10(-6) mol/L. (c) 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41499.
机译：生物传感器检测技术在过氧化氢的含量测定中引起了广泛的关注，过氧化氢已被非法用作食品添加剂。用壳聚糖固定的环糊精衍生物（6-CD-CTS）的C6-OH制备了一种检测H2O2的电化学生物传感膜。载体。通过在环糊精的疏水腔中包含二茂铁作为电子介体，并将过氧化氢酶（CAT）交联至6-CD-CTS的2-NH2，来制备生物传感器。通过紫外可见分光光度法和热重分析法评价包含二茂铁的配合物。还研究了其电化学行为。评价了反应条件对CAT固定化能力的影响。当使用以前的膜检测H2O2（C-H2O2）的浓度时，我们发现CAT的催化作用和二茂铁的信号放大对循环伏安图有重大影响。修饰电极的最佳工作pH为7.0。峰值电流（I）与H2O2浓度（CH2O2）在1.0 x 10（-4）到1.0 x 10（-3）mol / L之间具有线性关系。线性回归方程为I = 0.00475C（H2O2）-0.03025。检出限为10（-6）mol / L。 （c）2014 Wiley Periodicals，Inc. J. Appl。 Polym。科学2015，132，41499。

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