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首页> 外文期刊>Journal of Applied Polymer Science >Biodegradation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by a novel P3/4HB depolymerase purified from Agrobacterium sp DSGZ
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Biodegradation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by a novel P3/4HB depolymerase purified from Agrobacterium sp DSGZ

机译：由农杆菌sp DSGZ纯化的新型P3 / 4HB解聚酶对聚（3-羟基丁酸酯-co-4-羟基丁酸酯）的生物降解

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摘要

A poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P3/4HB)-degrading strain, Agrobacterium sp. DSGZ, was isolated from sewage by poly(3-hydroxybutyrate) (PHB) mineral agar plates. A novel P3/4HB depolymerase with a molecular weight of 34 kDa was purified through a novel single-step affinity chromatography method from the culture supernatant of the strain by using P3/4HB powder as a substrate. The purified depolymerase showed optimum activity at pH 7.0 and 50 degrees C, and was stable at the pH range of 6.0 to 9.0 and temperature below 50 degrees C. Enzyme activity was strongly inhibited by phenylmethylsulfonyl fluoride (PMSF), ethylenediaminetetraacetic acid (EDTA), hydrophobic reagents, and some metal ions. The depolymerase degraded poly(3-hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV), P3/4HB, and polycaprolactone (PCL), instead of polylactic acid (PLA) or poly(butylene succinate) (PBS). Meanwhile, the depolymerase showed high hydrolytic activity against short-chain length esters, such as butyrate acid ester and caprylic acid ester. The main degradation products of the depolymerase were identified as hydroxybutyrate monomers and dimers, and the monomers were identified as 3-hydroxybutyrate (3HB) monomers and 4-hydroxybutyrate (4HB) monomers. The preparation procedure, crystallinity, and 4HB composition of the P3/4HB copolymer showed evident effect on degradation behavior, and change in crystallinity was the main factor affecting degradation. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 42805.
机译：聚（3-羟基丁酸酯-co-4-羟基丁酸酯）（P3 / 4HB）降解菌株，农杆菌。通过聚（3-羟基丁酸）（PHB）矿物琼脂平板从污水中分离出DSGZ。以P3 / 4HB粉末为底物，通过新颖的一步亲和色谱法从菌株的培养上清液中纯化出分子量为34kDa的新型P3 / 4HB解聚酶。纯化的解聚酶在pH 7.0和50摄氏度下表现出最佳活性，并且在6.0到9.0的pH范围内以及低于50摄氏度的温度下均保持稳定。疏水试剂和一些金属离子。解聚酶降解聚（3-羟基丁酸酯）（PHB），聚（羟基丁酸酯-共-羟基戊酸酯）（PHBV），P3 / 4HB和聚己内酯（PCL），而不是聚乳酸（PLA）或聚丁二酸丁二酯（PBS） ）。同时，解聚酶对丁酸酯和辛酸酯等短链酯显示出高水解活性。解聚酶的主要降解产物被鉴定为羟基丁酸酯单体和二聚体，并且单体被鉴定为3-羟基丁酸酯（3HB）单体和4-羟基丁酸酯（4HB）单体。 P3 / 4HB共聚物的制备步骤，结晶度和4HB组成对降解行为有明显影响，结晶度的变化是影响降解的主要因素。 （c）2015 Wiley Periodicals，Inc. J. Appl。 Polym。科学2016，133，42805。

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