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首页> 外文期刊>Journal of Applied Polymer Science >Production of hyaluronic-acid-based cell-enclosing microparticles and microcapsules via enzymatic reaction using a microfluidic system
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Production of hyaluronic-acid-based cell-enclosing microparticles and microcapsules via enzymatic reaction using a microfluidic system

机译：通过使用微流体系统的酶促反应生产基于透明质酸的细胞包裹微粒和微胶囊

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This article describes the preparation of cell-enclosing hyaluronic acid (HA) microparticles with solid core and microcapsules with liquid core through cell-friendly horseradish peroxidase (HRP)-catalyzed hydrogelation. The spherical vehicles were made from HA derivative possessing phenolic hydroxyl moieties (HA-Ph) cross-linkable through the enzymatic reaction by extruding cell-suspending HA-Ph aqueous solution containing HRP from a needle of 180 m in inner diameter into the ambient coaxial flow of liquid paraffin containing H2O2 in a microtubule of 600 m in diameter. By altering the flow rate of liquid paraffin, the diameters of gelatin and HA-Ph microparticles were varied in the range of 120-220 m and 100-300 m, respectively. The viability of the enclosed human hepatoma HepG2 cells in the HA-Ph microparticles of 180 m in diameter was 94.2 +/- 2.3%. The growth of the enclosed HepG2 cells was enhanced by decreasing the HRP concentration. The microcapsules of 200 m in diameter were obtained by extruding HA-Ph aqueous solution containing thermally liquefiable cell-enclosing gelatin microparticles of 150 m in diameter using the same microfluidic system. The enclosed cells grew and filled the cavity within 10 days. Spherical tissues covered with a heterogeneous cell layer were obtained by degrading the microcapsule membrane using hyaluronidase after covering the surface with a heterogeneous cell layer. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43107.
机译：本文介绍了通过细胞友好型辣根过氧化物酶（HRP）催化的水凝胶化制备具有固体核心的带有细胞核的透明质酸（HA）微粒和具有液体核心的带有微囊的微胶囊的制备方法。球形载体由具有酚醛羟基部分（HA-Ph）的HA衍生物制成，可通过酶促反应从内径为180 m的针头中挤出含HRP的细胞悬浮HA-Ph水溶液，使其进入环境同轴流直径为600 m的微管中含有H2O2的液体石蜡的制备。通过改变液体石蜡的流速，明胶和HA-Ph微粒的直径分别在120-220m和100-300m的范围内变化。直径为180 m的HA-Ph微粒中封闭的人肝癌HepG2细胞的存活率为94.2 +/- 2.3％。降低HRP浓度可增强封闭的HepG2细胞的生长。通过使用相同的微流体系统挤出包含直径为150m的可热液化细胞的明胶微粒的HA-Ph水溶液，来获得直径为200m的微囊。封闭的细胞在10天内生长并充满空腔。在用异质细胞层覆盖表面之后，通过使用透明质酸酶降解微囊膜来获得被异质细胞层覆盖的球形组织。 （c）2015 Wiley Periodicals，Inc. J. Appl。 Polym。科学2016，133，43107。

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