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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection
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Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection

机译：基于质谱检测的亲水相互作用与离子对液相色谱法测定牛奶中链霉素和二氢链霉素的残留

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摘要

Streptomycin (STR) and dihydrostreptomycin (DHSTR) are two of the most common aminoglycoside antibiotics used in veterinary medicine. The physicochemical properties of both substances, make their determination challenging. In the present study the development of methods based on ion-pair chromatography (IPC) and on hydrophilic interaction chromatography (HILIC), for the determination of the above mentioned aminoglycosides in the range of 100-1000μgL~(-1) is described. The two methods were validated according to EU requirements for residues in food. The recoveries for the IPC method were 69.3% and 56.5% of STR and DHSTR, respectively, and for HILIC method 85.5% and 72.3%, respectively. The intra- and inter-day precision, studied at 100, 200 and 300μgkg~(-1) levels in milk samples, gave %RSD≤13 for both methods. LOQs for the HILIC method were 14μgkg~(-1) for both analytes and for the IPC method were 109 and 31μgkg~(-1), for STR and DHSTR, respectively. The sensitivity of the HILIC method is 80 and 210 times greater than that of the ICP method, for STR and DHSTR, respectively.
机译：链霉素（STR）和二氢链霉素（DHSTR）是兽药中最常用的两种氨基糖苷类抗生素。两种物质的物理化学性质都使其测定具有挑战性。在本研究中，描述了基于离子对色谱法（IPC）和亲水相互作用色谱法（HILIC）的方法的开发，用于测定上述100-1000μgL〜（-1）范围内的氨基糖苷。两种方法均根据欧盟对食品中残留的要求进行了验证。 IPC方法的回收率分别为STR和DHSTR的69.3％和56.5％，HILIC方法的回收率分别为85.5％和72.3％。在牛奶样品中分别以100、200和300μgkg〜（-1）的水平进行日内和日间精密度研究，两种方法的％RSD≤13。 HILIC方法的两种分析物的最低定量限为14μgkg〜（-1），IPC方法的STR和DHSTR的最低定量限分别为109和31μgkg〜（-1）。对于STR和DHSTR，HILIC方法的灵敏度分别比ICP方法高80倍和210倍。

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