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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Supercritical fluid extraction of microbial phospholipid fatty acids from activated sludge
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Supercritical fluid extraction of microbial phospholipid fatty acids from activated sludge

机译：超临界流体从活性污泥中提取微生物磷脂脂肪酸

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摘要

Supercritical carbon dioxide (scCO_2) extraction was applied for the determination of microbial phospholipid fatty acids (PLFA) in activated sludge. Quantification was performed by using gas chromatography-mass spectrometry (GC-MS). The highest extraction yields of PLFA, at a concentration of 7.28nmol/mg-dry activated sludge, was obtained at a temperature of 80°C, pressure of 25MPa and 10% (v/v) methanol for a 15-min extraction time. ScCO_2 extraction results obtained in these conditions were comparable with those obtained by liquid organic solvent extraction (LSE) based on diversity and equalibility indices. The repeatability test showed that the relative standard deviation values were less than 13%. The experimental results show that the scCO_2 extraction saves time and uses much less organic solvent. In addition, scCO_2 extraction is a promising and alternative method for the analysis of microbial community structure in environmental assessment using the PLFA profile.
机译：采用超临界二氧化碳（scCO_2）萃取法测定活性污泥中的微生物磷脂脂肪酸（PLFA）。通过使用气相色谱-质谱法（GC-MS）进行定量。在80°C的温度，25MPa的压力和10％（v / v）的甲醇中萃取15分钟，可获得最高浓度为7.28nmol / mg干活性污泥的PLFA提取率。在这些条件下获得的ScCO_2萃取结果与通过液体有机溶剂萃取（LSE）基于多样性和均等性指数获得的结果相当。重复性测试表明相对标准偏差值小于13％。实验结果表明，scCO_2萃取节省了时间，并且使用的有机溶剂少得多。此外，scCO_2提取是一种有前途的替代方法，可用于使用PLFA谱分析环境评估中的微生物群落结构。

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