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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >The frontiers of mass spectrometry-based techniques in food allergenomics
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The frontiers of mass spectrometry-based techniques in food allergenomics

机译：食品质谱中基于质谱技术的前沿

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In the last years proteomic science has started to provide an important contribution to the disclosure of basic aspects of food-related diseases. Among these, the identification of proteins involved in food allergy and their mechanism of activation of toxicity. Elucidation of these key issues requires the integration of clinical, immunological, genomic and proteomic approaches. These combined research efforts are aimed to obtain structural and functional information to assist the development of novel, more reliable and powerful diagnostic protocols alternative to the currently available procedures, mainly based on food challenge tests. Another crucial aspect related to food allergy is the need for methods to detect trace amounts of allergenic proteins in foods. Mass spectrometry is the only non-immunological method for high-specificity and high-sensitivity detection of allergens in foods. Nowadays, once provided the appropriate sample handling and the correct operative conditions, qualitative and quantitative determination of allergens in foods and ingredients can be efficiently obtained by MALDI-TOF-MS and LC-MS/MS methods, with limits of detection and quantification in the low-ppb range. The availability of accurate and fast alternatives to immunological ELISA tests may also enable the development of novel therapeutic strategies and food processing technologies to aid patients with food allergy or intolerance, and to support allergen labelling and certification processes, all issues where the role of proteomic science is emerging.
机译：近年来，蛋白质组学已开始为揭示与食物相关疾病的基本方面做出重要贡献。其中，涉及食物过敏的蛋白质及其毒性激活机制的鉴定。要阐明这些关键问题，需要整合临床，免疫学，基因组和蛋白质组学方法。这些综合的研究工作旨在获得结构和功能信息，以协助开发新颖，更可靠，功能更强大的诊断方案，以替代现有的程序（主要基于食物挑战试验）。与食物过敏有关的另一个关键方面是需要检测食物中痕量致敏蛋白的方法。质谱法是用于食品中过敏原高特异性和高灵敏度检测的唯一非免疫方法。如今，一旦提供了适当的样品处理方法和正确的操作条件，就可以通过MALDI-TOF-MS和LC-MS / MS方法有效地定性和定量地测定食品和配料中的过敏原，而且检测和定量的极限低ppb范围。准确，快速的替代方法可以替代免疫学ELISA测试，还可以开发新颖的治疗策略和食品加工技术，以帮助患有食物过敏或不耐受的患者，并支持过敏原标签和认证流程，这是蛋白质组学在蛋白质组学中的作用正在兴起。

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