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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Comparison of void volume, mobile phase volume and accessible volume determined from retention data for oligomers in reversed-phase liquid chromatographic systems
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Comparison of void volume, mobile phase volume and accessible volume determined from retention data for oligomers in reversed-phase liquid chromatographic systems

机译：根据反相液相色谱系统中低聚物保留数据确定的空隙体积，流动相体积和可及体积的比较

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摘要

The experimental technique of mass spectrometric tracer pulse chromatography was used to determine the void volume, i.e., the total volume of eluent in the column, and the volume of eluent moving freely through the column, i.e., mobile phase volume, for a series of eluents with a C_(18)-bonded RPLC column. The interstitial volume of the column was determined by size exclusion chromatography. In order to evaluate the utility of the accessible volumes determined from the retention volumes of homologous solutes, the accessible volume of the column was determined as a function of eluent composition and temperature with polystyrene and polyethylene glycol samples using Martin's Rule. Comparison of these four measured volumes indicated that the experimentally measured accessible volumes did not correspond to either the void volumes, mobile phase volumes or interstitial volumes.
机译：质谱示踪脉冲色谱法的实验技术用于确定一系列洗脱液的空隙体积，即色谱柱中洗脱液的总体积，以及洗脱液自由流过色谱柱的体积，即流动相体积具有C_（18）键合的RPLC色谱柱。通过尺寸排阻色谱法确定柱的间隙体积。为了评估由同源溶质的保留体积确定的可及体积的实用性，使用马丁定律，根据聚苯乙烯和聚乙二醇样品的洗脱液组成和温度确定色谱柱的可及体积。对这四个测量体积的比较表明，实验测量的可及体积与空隙体积，流动相体积或间隙体积都不对应。

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