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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Investigations on the chromatographic behaviour of zwitterionic stationary phases used in hydrophilic interaction chromatography
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Investigations on the chromatographic behaviour of zwitterionic stationary phases used in hydrophilic interaction chromatography

机译：亲水相互作用色谱中两性离子固定相的色谱行为研究

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摘要

Two commercial stationary phases possessing a sulfobetaine zwitterionic bonded ligand (ZIC-HILIC and Nucleodur HILIC) were compared under hydrophilic interaction chromatographic (HILIC) conditions. First of all, the separation of 12 model compounds chosen among neurotransmitters and presenting a diversity of ionization states (anionic, cationic and zwitterionic) was studied under varied operating conditions. The effects of the percentage of acetonitrile, ammonium acetate concentration and temperature of the mobile phase were compared on the two columns. Secondly, a generally applicable retention model was established, based on chromatographic retention data (log. k) acquired for 76 model compounds. The chosen compounds are small molecules presenting a wide diversity of molecular structures and are relevant to biomedical and pharmaceutical studies. To account for their retention behaviour, a modified version of the solvation parameter model was designed: two additional molecular descriptors were introduced, to account for ionic interactions with anionic and cationic species. The retention equations obtained allow a rationalization of the interactions contributing to retention and separation in the HILIC systems considered.
机译：在亲水作用色谱（HILIC）条件下，比较了具有磺基甜菜碱两性离子键合配体的两个商业固定相（ZIC-HILIC和Nucleodur HILIC）。首先，研究了在各种操作条件下，从神经递质中选择的12种模型化合物的分离方法，这些化合物具有多种电离状态（阴离子，阳离子和两性离子）。在两根色谱柱上比较了乙腈百分比，乙酸铵浓度和流动相温度的影响。其次，基于对76种模型化合物获得的色谱保留数据（log.k），建立了通用的保留模型。选择的化合物是小分子，具有广泛的分子结构，与生物医学和药物研究有关。为了说明其保留行为，设计了溶剂化参数模型的修改版本：引入了两个附加的分子描述符，以说明离子与阴离子和阳离子物质的相互作用。所获得的保留方程可以合理化相互作用，从而有助于所考虑的HILIC系统中的保留和分离。

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