...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Mobile phase effects in reversed-phase liquid chromatography: A comparison of acetonitrile/water and methanol/water solvents as studied by molecular simulation
【24h】

Mobile phase effects in reversed-phase liquid chromatography: A comparison of acetonitrile/water and methanol/water solvents as studied by molecular simulation

机译：反相液相色谱中的流动相效应：通过分子模拟研究乙腈/水和甲醇/水溶剂的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Molecular simulations of water/acetonitrile and water/methanol mobile phases in contact with a C_(18) stationary phase were carried out to examine the molecular-level effects of mobile phase composition on structure and retention in reversed-phase liquid chromatography. The simulations indicate that increases in the fraction of organic modifier increase the amount of solvent penetration into the stationary phase and that this intercalated solvent increases chain alignment. This effect is slightly more apparent for acetonitrile containing solvents. The retention mechanism of alkane solutes showed contributions from both partitioning and adsorption. Despite changes in chain structure and solvation, the molecular mechanism of retention for alkane solutes was not affected by solvent composition. The mechanism of retention for alcohol solutes was primarily adsorption at the interface between the mobile and stationary phase, but there were also contributions from interactions with surface silanols. The interaction between the solute and surface silanols become very important at high concentrations of acetonitrile.
机译：进行了水/乙腈和水/甲醇流动相与C_（18）固定相接触的分子模拟，以研究流动相组成对反相液相色谱法对结构和保留率的分子水平影响。模拟表明，有机改性剂比例的增加会增加溶剂渗透进固定相的量，并且这种插层的溶剂会增加链的排列。对于含乙腈的溶剂，这种作用稍显明显。烷烃溶质的保留机理显示出来自分配和吸附的贡献。尽管链结构和溶剂化发生了变化，但烷烃溶质保留的分子机理不受溶剂组成的影响。醇溶质的保留机理主要是在流动相和固定相之间的界面吸附，但也有与表面硅烷醇相互作用的贡献。在高浓度的乙腈中，溶质与表面硅烷醇之间的相互作用变得非常重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号