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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Determination of methotrexate and folic acid by ion chromatography with electrochemical detection on a functionalized multi-wall carbon nanotube modified electrode
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Determination of methotrexate and folic acid by ion chromatography with electrochemical detection on a functionalized multi-wall carbon nanotube modified electrode

机译：离子色谱-功能化多壁碳纳米管修饰电极电化学检测法测定甲氨蝶呤和叶酸

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摘要

A simple and sensitive method was developed for simultaneous determination of methotrexate (MTX) and folic acid (FA) by ion chromatography with electrochemical detection (IC-ECD). Quaternary amine functionalized multi-wall carbon nanotubes (q-MWNTs) modified glassy carbon electrode (GCE) was used as an amperometric sensor to determine MTX and FA. The electrochemical behaviors of MTX and FA at the q-MWNTs/GCE were studied by cyclic voltammetry (CV). Results indicated that this modified electrode exhibited electrocatalytic oxidation toward MTX and FA with high sensitivity, stability and long life. Good separation of MTX and FA was demonstrated by IC on an anion-exchange column with phosphate buffer solution (PBS) as eluent. Under the optimal conditions, the linear ranges were from 0.01 to 20. mg/L for both MTX and FA with correlation coefficients r≥. 0.9994. The obtained detection limits (LODs) for MTX and FA were 0.2 and 0.4 μg/L (S/. N=. 3), respectively. The method has been successfully applied to the determination of MTX and FA in plasma and urine of patients of rheumatoid arthritis.
机译：建立了一种简单而灵敏的方法，可通过离子色谱-电化学检测（IC-ECD）同时测定甲氨蝶呤（MTX）和叶酸（FA）。季胺官能化的多壁碳纳米管（q-MWNTs）修饰的玻碳电极（GCE）被用作安培传感器，以确定MTX和FA。通过循环伏安法（CV）研究了MTX和FA在q-MWNTs / GCE上的电化学行为。结果表明，该修饰电极对MTX和FA具有电催化氧化作用，灵敏度高，稳定性好，寿命长。 IC在阴离子交换柱上用磷酸盐缓冲溶液（PBS）作为洗脱液证实了MTX和FA的良好分离。在最佳条件下，MTX和FA的线性范围均为0.01至20 mg / L，相关系数r≥。 0.9994。获得的MTX和FA的检出限（LOD）分别为0.2和0.4μg/ L（S /。N =。3）。该方法已成功应用于类风湿关节炎患者血浆和尿液中MTX和FA的测定。

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