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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Partition efficiencies of newly fabricated universal high-speed counter-current chromatograph for separation of two different types of sugar derivatives with organic-aqueous two-phase solvent systems
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Partition efficiencies of newly fabricated universal high-speed counter-current chromatograph for separation of two different types of sugar derivatives with organic-aqueous two-phase solvent systems

机译：新型通用高速逆流色谱仪的分离效率，可通过有机水两相溶剂系统分离两种不同类型的糖衍生物

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A new design of universal high-speed counter-current chromatograph (HSCCC) was fabricated in our laboratory. It holds a set of four column holders symmetrically around the rotary frame at a distance of 11.2 cm from the central axis. By engaging the stationary gear on the central axis of the centrifuge to the planetary gears on the column holder shaft through a set of idle gears, two pairs of diagonally located column holders simultaneously rotate about their own axes in the opposite directions: one forward (type-J planetary motion) and the other backward (type-I planetary motion) each synchronously with the revolution. Using the eccentric coil assembly, partition efficiencies produced by these two planetary motions were compared on the separation of two different types of sugar derivatives (4-methylumbelliferyl and 5-bromo-4-chloro-3-indoxyl sugar derivatives) using organic-aqueous two-phase solvent systems composed of n-hexane/ethyl acetate/1-butanol/methanol/water and aqueous 0.1 M sodium tetraborate, respectively. With lower phase mobile, better peak resolution was obtained by the type-J forward rotation for both samples probably due to higher retention of the stationary phase. With upper phase mobile, however, similar peak resolutions were obtained between these two planetary motions for both sugar derivatives. The overall results indicate that the present universal HSCCC is useful for counter-current chromatographic separation since each planetary motion has its specific applications: e.g., vortex CCC by the type-I planetary motion and HSCCC by the type-J planetary motion both for separation of various natural and synthetic products.
机译：我们实验室制造了一种新的通用高速逆流色谱仪（HSCCC）。它在旋转框架周围与中心轴相距11.2 cm的位置对称地对称地支撑着一组四个色谱柱支架。通过一组空转齿轮将离心机中心轴上的固定齿轮与色谱柱固定器轴上的行星齿轮啮合，两对斜置的色谱柱固定器同时沿各自的轴线沿相反方向旋转：一个向前（类型-J行星运动）和另一个后退（I型行星运动）分别与公转同步。使用偏心线圈组件，比较了使用两种有机水溶液分离两种不同类型的糖衍生物（4-甲基伞形糖和5-溴-4-氯-3-吲哚糖衍生物）时，这两种行星运动产生的分配效率。相溶剂体系分别由正己烷/乙酸乙酯/ 1-丁醇/甲醇/水和0.1 M四硼酸钠水溶液组成。使用较低的相移动量，两个样品的J型正向旋转可获得更好的峰分离度，这可能是由于固定相的保留较高。然而，利用上相移动，两种糖衍生物在这两个行星运动之间获得了相似的峰分辨率。总体结果表明，当前的通用HSCCC可用于逆流色谱分离，因为每个行星运动都有其特定的应用：例如，通过I型行星运动产生的涡旋CCC和通过J型行星运动产生的HSCCC都可以分离出各种天然和合成产品。

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