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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry
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Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry

机译：基于液滴的液体微结表面采样-高效液相色谱-电喷雾电离串联质谱法定量测定小鼠全身薄组织切片中的瑞格列奈和代谢物

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Herein, quantitation aspects of a fully automated autosampler/HPLC-MS/MS system applied for unattended droplet-based surface sampling of repaglinide dosed thin tissue sections with subsequent HPLC separation and mass spectrometric analysis of parent drug and various drug metabolites were studied. Major organs (brain, lung, liver, kidney and muscle) from whole-body thin tissue sections and corresponding organ homogenates prepared from repaglinide dosed mice were sampled by surface sampling and by bulk extraction, respectively, and analyzed by HPLC-MS/MS. A semi-quantitative agreement between data obtained by surface sampling and that by employing organ homogenate extraction was observed. Drug concentrations obtained by the two methods followed the same patterns for post-dose time points (0.25, 0.5, 1 and 2h). Drug amounts determined in the specific tissues was typically higher when analyzing extracts from the organ homogenates. In addition, relative comparison of the levels of individual metabolites between the two analytical methods also revealed good semi-quantitative agreement. (C) 2015 Elsevier B.V. All rights reserved.
机译：在本文中，研究了全自动自动进样器/ HPLC-MS / MS系统的定量方面，该系统用于瑞格列奈剂量薄组织切片的无人值守基于液滴的表面采样，随后进行了HPLC分离以及母体药物和各种药物代谢物的质谱分析。分别通过表面采样和批量提取对来自全身薄组织切片的主要器官（脑，肺，肝，肾和肌肉）和由瑞格列奈给药的小鼠制备的相应器官匀浆进行采样，并通过HPLC-MS / MS进行分析。观察到通过表面采样获得的数据与采用器官匀浆提取的数据之间的半定量一致性。通过两种方法获得的药物浓度在给药后的时间点（0.25、0.5、1和2h）遵循相同的模式。当分析来自器官匀浆的提取物时，在特定组织中测定的药物量通常较高。此外，两种分析方法之间单个代谢物水平的相对比较也显示出良好的半定量一致性。 （C）2015 Elsevier B.V.保留所有权利。

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