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首页> 外文期刊>Journal of Geophysical Research. Biogeosciences >Shifts in the community structure and activity of anaerobic ammonium oxidation bacteria along an estuarine salinity gradient
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Shifts in the community structure and activity of anaerobic ammonium oxidation bacteria along an estuarine salinity gradient

机译：厌氧铵氧化细菌的群落结构和活性沿河口盐度梯度的变化

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摘要

Anaerobic ammoniumoxidation (anammox) is amajormicrobial pathway for nitrogen (N) removal in estuarine and coastal environments. However, understanding of anammox bacterial dynamics and associations with anammox activity remains scarce along estuarine salinity gradient. In this study, the diversity, abundance, and activity of anammox bacteria, and their potential contributions to total N_2 production in the sediments along the salinity gradient (0.1–33.8) of the Yangtze estuarine and coastal zone, were studied using 16S rRNA gene clone library, quantitative polymerase chain reaction assay, and isotope-tracing technique. Phylogenetic analysis showed a significant change in anammox bacterial community structure along the salinity gradient (P<0.01), with the dominant genus shifting from Brocadia in the freshwater region to Scalindua in the open ocean. Anammox bacterial abundance ranged from 3.67× 10~5 to 8.22 × 10~7 copies 16S rRNA gene g~(-1) and related significantly with salinity (P<0.05). The anammox activity varied between 0.08 and 6.46nmolNg~(-1) h~(-1) and related closely with anammox bacterial abundance (P<0.01). Contributions of anammox activity to total N loss were highly variable along the salinity gradient, ranging from 5 to 77% and were significantly negatively correlated with salinity (P<0.01). Sediment organicmatter was also recognized as an important factor in controlling the relative role of anammox to total N2 production in the Yangtze estuarine and coastal zone. Overall, our data demonstrated a biogeographical distribution of anammox bacterial diversity, abundance, and activity along the estuarine salinity gradient and suggested that salinity is a major environmental control on anammox process in the estuarine and coastal ecosystems.
机译：厌氧氨氧化（厌氧氨氧化）是河口和沿海环境中氮（N）去除的主要微生物途径。然而，沿河口盐度梯度对厌氧氨氧化细菌动力学及其与厌氧氨氧化活性的关系的了解仍然很少。在这项研究中，利用16S rRNA基因克隆研究了厌氧细菌的多样性，丰度和活性，以及​​它们对长江口和沿海地区盐度梯度（0.1–33.8）沉积物中总N_2产生的潜在贡献。文库，定量聚合酶链反应分析和同位素示踪技术。系统发育分析表明，沿盐度梯度，厌氧细菌群落结构发生了显着变化（P <0.01），优势菌从淡水地区的布罗卡迪亚转移到公海的斯卡林达。厌氧菌的细菌丰度范围为3.67×10〜5到8.22×10〜7个拷贝的16S rRNA基因g〜（-1），与盐度显着相关（P <0.05）。厌氧菌活性在0.08〜6.46nmolNg〜（-1）h〜（-1）之间变化，与厌氧菌的细菌丰度密切相关（P <0.01）。厌氧氨氧化活性对总氮损失的贡献在盐度梯度上变化很大，范围为5-77％，并且与盐度显着负相关（P <0.01）。沉积物有机质也被认为是控制长江河口和沿海地区厌氧菌对总N2产生的相对作用的重要因素。总体而言，我们的数据显示了沿河口盐度梯度的厌氧细菌多样性，丰度和活性的生物地理分布，并表明盐度是河口和沿海生态系统中厌氧氨化过程的主要环境控制。

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