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首页> 外文期刊>Journal of Materials Science >Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications
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Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications

机译：PCL /明胶/壳聚糖三元纳米纤维复合支架的制备与表征

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摘要

In the present study, we have fabricated a ternary composite nanofibrous scaffold from PCL/gelatin/ chitosan, by electrospinning technique, using a solvent system—chloroform/methanol for polycaprolactone (PCL) and acetic acid for gelatin and chitosan, for tissue engineering applications. Field emission scanning electron microscopy (FE-SEM) was used to investigate the fiber morphology of the scaffold and it was found that the fiber morphology was influenced by the concentrations of PCL, gelatin, and chitosan in polymer solution during electrospinning. X-ray diffraction, Fourier transform infrared, and thermogravimetric (TG) analysis results showed some interactions among the molecules of PCL, gelatin, and chitosan within the scaffold. In-vitro cell culture studies were done by seeding L929 mouse fibroblasts on fabricated composite scaffold, which confirmed the cell viability, high cell proliferation rate, and cell adhesion on composite scaffold as indicated by MTT assay, DNA quantification, and FE-SEM analysis of cell-scaffold construct. Thus, the ternary composite scaffold made from the combination of PCL (synthetic polymer), gelatin, and chitosan (natural polymer) may find potential application in tissue engineering.
机译：在本研究中，我们通过静电纺丝技术，使用氯仿/甲醇制聚己内酯（PCL）和乙酸制明胶和壳聚糖，通过电纺技术从PCL /明胶/壳聚糖制备了三元复合纳米纤维支架，用于组织工程应用。用场发射扫描电子显微镜（FE-SEM）研究了支架的纤维形态，发现在静电纺丝过程中，纤维形态受到聚合物溶液中PCL，明胶和壳聚糖浓度的影响。 X射线衍射，傅立叶变换红外和热重（TG）分析结果表明，支架中PCL，明胶和壳聚糖分子之间存在一些相互作用。体外细胞培养研究是通过将L929小鼠成纤维细胞接种在已制成的复合支架上进行的，这证实了细胞活力，高细胞增殖速率和在复合支架上的细胞粘附性，如MTT分析，DNA定量和FE-SEM分析所表明的那样。细胞支架构建体。因此，由PCL（合成聚合物），明胶和壳聚糖（天然聚合物）的组合制成的三元复合支架可能在组织工程中具有潜在的应用。

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