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首页> 外文期刊>Journal of Molecular Biology >THE STRUCTURE OF A NOVEL DNA DUPLEX FORMED BY HUMAN CENTROMERE D(TGGAA) REPEATS WITH POSSIBLE IMPLICATIONS FOR CHROMOSOME ATTACHMENT DURING MITOSIS
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THE STRUCTURE OF A NOVEL DNA DUPLEX FORMED BY HUMAN CENTROMERE D(TGGAA) REPEATS WITH POSSIBLE IMPLICATIONS FOR CHROMOSOME ATTACHMENT DURING MITOSIS

机译：人三角洲D（TGGAA）重复形成的新型DNA双链体的结构，可能在减数分裂过程中影响染色体的附着

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摘要

The solution structure of the DNA duplex [GTGGAATGGAAC](2) containing a tandem repeat of the human centromere (TGGAA)(n) unit has been determined by two-dimensional nuclear magnetic resonance (2D-NMR), distance geometry (DG) and molecular dynamics/energy minimization (MD/EM) methods. This remarkably stable ''self-complementary'' antiparallel duplex contains a tandem repeated motif in which unpaired guanine residues from opposite strands intercalate and costack between sheared G . A pairs. Twelve independent refined structures were determined from the NMR data and found to converge to a single family of closely related structures with pair-wise r.m.s.d. values of 0.55 +/- 0.25 Angstrom. All sugar residues are in the normal C2'-endo conformation except for the unpaired guanosines, which are in the unusual C3'-endo conformation. The guanosine residues of the bracketing G . A pairs have high-antiglycosidic torsion angles and zeta backbone torsion angles close to the trans domain. The structure exhibits many unusual interstrand interactions, including base-sugar stacking, base-phosphate hydrogen bonding and cross-strand base stacking. The [GGA](2) unit contains a stack of four contiguous guanine residues, all of which have their hydrogen-bonding surface ((NH)-H-2-N1H-O(6)N7) exposed to solvent and available for interaction with other bases or ligands. This unexpected property may explain the unique morphology and function of the human centromere in mitosis. (C) 1995 Academic Press Limited [References: 43]
机译：包含人类着丝粒（TGGAA）（n）单元串联重复序列的DNA双链体[GTGGAATGGAAC]（2）的溶液结构已通过二维核磁共振（2D-NMR），距离几何结构（DG）和分子动力学/能量最小化（MD / EM）方法。这种非常稳定的“自我互补”反平行双链体包含一个串联重复基序，其中来自相反链的未配对鸟嘌呤残基插入并在剪切的G之间形成哥斯达克。一双。从NMR数据确定了十二个独立的精制结构，发现它们收敛到具有成对r.m.s.d的紧密相关结构的单个家族。值为0.55 +/- 0.25埃。除未配对的鸟苷外，所有糖残基均处于正常的C2'-内构象，而鸟苷则具有不寻常的C3'-内构象。包围G的鸟苷残基。一对具有接近反式结构域的高抗糖苷扭转角和zeta主链扭转角。该结构表现出许多异常的链间相互作用，包括碱基糖堆叠，碱基磷酸氢键和交叉链碱基堆叠。 [GGA]（2）单元包含四个相邻的鸟嘌呤残基的堆栈，所有这些残基的氢键表面（（NH）-H-2-N1H-O（6）N7）暴露于溶剂中且可相互作用与其他碱基或配体。这种意外的特性可能解释了人类着丝粒在有丝分裂中的独特形态和功能。 （C）1995 Academic Press Limited [参考：43]

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