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首页> 外文期刊>Journal of Molecular Biology >The nucleoside triphosphate diphosphohydrolase-1/CD39 is incorporated into human immunodeficiency type 1 particles, where it remains biologically active
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The nucleoside triphosphate diphosphohydrolase-1/CD39 is incorporated into human immunodeficiency type 1 particles, where it remains biologically active

机译：核苷三磷酸二磷酸水解酶-1 / CD39被掺入人类免疫缺陷1型颗粒，并在其中保持生物活性

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摘要

Human immunodeficiency virus type I (HIV-1) carries a variety of host proteins in addition to virus-encoded structural proteins, both in its envelope and inside the viral particle. Previous studies have reported that the HIV-1 life-cycle is affected by such virus-associated host cell surface proteins. The nucleoside triphosphate diphosphohydrolase-1 (NTPDasel), also known as CD39, is a plasma membrane-bound ectoenzyme that hydrolyzes extracellular ATP and ADP to AMP. It has been shown that CD39 inhibits platelet function, and is thus a critical thromboregulatory molecule. We demonstrate here that host-derived CD39 is acquired by both laboratory-adapted and clinical variants of HIV-1 produced in cellular reservoirs of the virus. Moreover, purified CD39-bearing virions, but not isogenic viruses lacking CD39, display strong ATPase and ADPase activities. It is of particular interest that virions bearing this cellular enzyme can inhibit ADP-induced platelet aggregation, an effect blocked by an NTPDase inhibitor. On the basis of published and the present data on the functionality of human cellular proteins embedded within HIV-1, it can be proposed that these proteins might contribute to some of the immunologic deficiencies seen in infected individuals. (c) 2007 Elsevier Ltd. All rights reserved.
机译：I型人类免疫缺陷病毒（HIV-1）除了被病毒编码的结构蛋白外，还在其包膜和病毒颗粒内部还携带多种宿主蛋白​​。先前的研究报道了HIV-1的生命周期受到这种病毒相关宿主细胞表面蛋白的影响。核苷三磷酸二磷酸水解酶1（NTPDasel），也称为CD39，是一种质膜结合的外切酶，可将细胞外ATP和ADP水解为AMP。已经显示CD39抑制血小板功能，因此是关键的血栓调节分子。我们在这里证明宿主衍生的CD39是通过病毒的细胞贮库中产生的HIV-1实验室适应和临床变体获得的。此外，纯化的带有CD39的病毒体，但没有缺少CD39的同基因病毒，显示出强大的ATPase和ADPase活性。特别令人感兴趣的是，带有这种细胞酶的病毒体可以抑制ADP诱导的血小板凝集，这种作用被NTPDase抑制剂阻断。根据有关嵌入HIV-1的人类细胞蛋白功能的公开和当前数据，可以提出这些蛋白可能会导致感染个体中某些免疫缺陷。 （c）2007 Elsevier Ltd.保留所有权利。

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