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首页> 外文期刊>Journal of Molecular Biology >The Multiple Roles of cyclin E1 in Controlling Cell Cycle Progression and Cellular Morphology of Trypanosoma brucei.
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The Multiple Roles of cyclin E1 in Controlling Cell Cycle Progression and Cellular Morphology of Trypanosoma brucei.

机译：细胞周期蛋白E1在控制布鲁氏锥虫细胞周期进程和细胞形态学中的多重作用。

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Regulation of eukaryotic cell cycle progression requires sequential activation and inactivation of cyclin-dependent kinases. Previous RNA interference (RNAi) experiments in Trypanosoma brucei indicated that cyclin E1, cdc2-related kinase (CRK)1 and CRK2 are involved in regulating G1/S transition, whereas cyclin B2 and CRK3 play a pivotal role in controlling the G2/M checkpoint. To search for potential interactions between the other cyclins and CRKs that may not have been revealed by the RNAi assays, we used the yeast two-hybrid system and an in vitro glutathione-S-transferase pulldown assay and observed interactions between cyclin E1 and CRK1, CRK2 and CRK3. Cyclins E1-E4 are homologues of yeast Pho80 cyclin. But yeast complementation assays indicated that none of them possesses a Pho80-like function. Analysis of cyclin E1+CRK1 and cyclin E1+CRK2 double knockdowns in the procyclic form of T. brucei indicated that the cells were arrested more extensively in the G1 phase beyond the cumulative effect of individual knockdowns. But BrdU incorporation was impaired significantly only in cyclin E1+CRK1-depleted cells, whereas a higher percentage of cyclin E1+CRK2 knockdown cells assumed a grossly elongated posterior end morphology. A double knockdown of cyclin E1 and CRK3 arrested cells in G2/M much more efficiently than if only CRK3 was depleted. Taken together, these data suggest multiple functions of cyclin E1: it forms a complex with CRK1 in promoting G1/S phase transition; it forms a complex with CRK2 in controlling the posterior morphogenesis during G1/S transition; and it forms a complex with CRK3 in promoting passage across the G2/M checkpoint in the trypanosome.
机译：调节真核细胞周期进程需要细胞周期蛋白依赖性激酶的顺序激活和失活。布鲁氏锥虫先前的RNA干扰（RNAi）实验表明，细胞周期蛋白E1，cdc2相关激酶（CRK）1和CRK2参与调节G1 / S的转变，而细胞周期蛋白B2和CRK3在控制G2 / M检查点中起关键作用。为了寻找RNAi分析未发现的其他细胞周期蛋白和CRK之间的潜在相互作用，我们使用了酵母双杂交系统和体外谷胱甘肽-S-转移酶下拉实验，观察到细胞周期蛋白E1和CRK1之间的相互作用， CRK2和CRK3。细胞周期蛋白E1-E4是酵母Pho80细胞周期蛋白的同源物。但是酵母互补分析表明它们都不具有Pho80样功能。对布鲁氏杆菌的前环形式的细胞周期蛋白E1 + CRK1和细胞周期蛋白E1 + CRK2双重敲除的分析表明，除个别敲除的累积作用外，细胞在G1期的捕获更为广泛。但是，BrdU掺入仅在细胞周期蛋白E1 + CRK1缺失的细胞中显着受损，而较高百分比的细胞周期蛋白E1 + CRK2敲低细胞则认为其后端形态显着延长。与仅消耗CRK3相比，双重敲除cyclin E1和CRK3可以更有效地阻滞G2 / M细胞。综上所述，这些数据表明细胞周期蛋白E1具有多种功能：它与CRK1形成复合物，促进G1 / S相变;它与CRK2形成复合物，以控制G1 / S过渡过程中的后形态发生。它与CRK3形成复合物，促进锥虫体中G2 / M检查点的通过。

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